The evaluation of a method for the estimation of serum urate using immobilized uricase is described, the resultant hydrogen peroxide produced being measured by the oxidative coupling of 3,5-dichloro-2-hydroxybenzenesulfonate and 4-aminophenazone in the presence of peroxidase.A continuous-flow analysis system incorporating the uricase tube was established, and the results obtained were correlated with an automated phosphotungstate method and with a manual uricase method employing an LKB 8600 Rate Reaction Analyser. The effect of ascorbic acid on the analysis of serum urate and the elimination of this interference by the use of ascorbate oxidase was also investigated.The precision, correlation, and high specificity obtained show that this is a satisfactory method for use in routine clinical laboratory works.
Immobilized enzyme nylon-tube reactors incorporating creatinine iminohyrolase (CI) and glutamate dehydrogenase (GDH) were used to assay creatinine in serum and urine. Optimum substrate concentrations for the assay were determined. The reactors were incorporated into a continuous flow system for creatinine analysis. The method was evaluated with respect to linearity, sample interaction, precision, accuracy, and analytical recovery. Comparison studies were carried out with a standard Jaffé method and the effect of interfering substances was investigated. From the results obtained, it was concluded that the assay was suitable as a simple, reliable, and specific method for serum and urine creatinine determinations.
A single immobilized enzyme nylon tube reactor was produced incorporating a four enzyme system for the analysis of creatinine. The enzyme activity ratios in the coupling solution used to prepare the reactor were found to be of extreme importance in governing the activity of the latter. The reactor was incorporated into a continuous flow analysis system used to assay creatinine in urine samples and the results were correlated with a manual technique employing the same enzyme system in solution. The precision, correlation, high specificity, simplicity, and speed of the analysis were concluded to be factors in favor of the method's suitability for urine creatinine determinations.
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