This is an author produced version of a paper published in Urban greenspace has consistently been argued to be of great importance to the wellbeing, health, and daily lives of residents and users. This paper reports results from a study which combined the visualisation of public greenspace with environmental economics, and which aimed to develop a method by which realistic computer models of sites could be used within preference studies. As part of a methodology which employed contingent rating to establish the values placed on specific greenspace sites, three-dimensional computer models were used to produce visualisations of particular environmental conditions. Of particular importance to the study was the influence of variables including lighting, season, time of day and weather on the perception of respondents. This study followed previous work that established a suitable approach to the modelling and testing of entirely moveable physical variables within the built environment. As such, the study has firmly established that computer-generated visualisations are appropriate for use within environmental economic surveys, and that there is potential for a holistic range of attributes to be included in such studies.
The putative gene encoding acetyl-CoA carboxylase, accA, has been isolated from Aspergillus nidulans. This single-copy gene has an open reading frame (ORF) of 6864 bp and contains two small introns near the 5'-end. A short ORF upstream of the ATG start codon has been identified in this gene by RT-PCR. Based on sequence homology to acetyl-CoA carboxylases from other organisms, putative biotin-, ATP-, HCO3-- and acetyl-CoA- binding sites have been assigned. Northern data and ACC enzyme-activity measurements from A. nidulans suggested that expression of accA was higher in media containing nitrate than ammonia as a sole nitrogen source. Deletion of accA in A. nidulans was unsuccessful. The failure of A. nidulans to grow in the presence of the ACC-specific inhibitor, soraphen A, supplemented with C16-18 fatty acids suggested that ACC is an essential enzyme.
Modern agricultural practices have been strongly linked with increased N03-N loadings in surface waters. Nitrate leaching increases as land use progresses from forest and moorland through grassland, to arable agriculture. There are, within the UK, few studies on a regional scale capable of displaying a relationship between land cover (agricultural intensity) and water quality. This relationship can be investigated using computer manipulation of spatial geographic information together with conventional river and agricultural census data.Simple regression analysis against primary land cover suggests that agriculture is reponsible for annual losses of nitrate in North East Scotland river catchments. Further multi-linear regression analysis, using the GIS data and agricultural census returns indicate that most of the outstanding variation can be accounted for if the agricultural variable is related to agricultural practice, such as spring, winter and grass cropping.
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