The transition monopole theory of electronic excitation interaction is described and applied to the theory of resonance transfer for chlorophyll molecules. It is then compared with analogous results based on transition dipole theory. The correction to the Förster theory of resonance transfer is evaluated in various geometries for the case of short range transfer. The enhancement or reduction factor for the transfer rate in various chlorophylls is of the order of two to one tenth for the range that we are interested in, i.e., 12–30 Å, and anisotropy of the transfer rate is greatly increased. A possible enhancement of the transfer rates in the case of forbidden transitions in porphin is discussed.
Theoretical investigations of electronic distributions in eight different structural forms of nitrosylhemoglobin were carried out to study the changes in 14N hyperfine interaction observed with the transition from R to T structures under the influence of inositol hexaphosphate or changing pH. Four of the eight forms studied consisted of protonated and deprotonated N ros in the proximal imidazole ligand with linear and bent Fe-N-0 structures. Two other forms had a straight Fe-N-O structure and Fe-Im bond stretched by 0.5 and 1.0 .A. The other two systems we have studied are five-liganded NO-heme with bent and straight Fe-N-O structures. Our investigations show that arrangements of energy levels did not differ significantly among all the structures, the unpaired electron always occupying an antibonding orbital with d.2 symmetry. The protonated and deprotonated systems with either linear or bent Fe-N-O structure showed substantial hyperfine interaction of the 14N nuclei of the NO group and the Nf atom of the proximal imidazole, indicating that a 9-line electron spin resonance hyperfine pattern (R structure) would be expected in all four cases. On the other hand, the extensions of the Fe-Im bond produce a sizeable decrease in the 14Nf hyperfine interaction, indicating that an extension beyond 1.0 A\ would provide a 3-line hyperfine pattern close to that found for the five-liganded NO-heme system. Our results thus provide quantitative support for the model of severe extension or cleavage of the Fe-N, bond proposed in the literature for explaining the R-to-T transition of the a-chain of nitrosylhemoglobin.The study of the properties of nitrosylhemoglobin (NO-Hb) has assumed major importance (1-4) in recent years for two main reasons. First, it is a six-liganded hemoglobin derivative that is paramagnetic with spin 1/2, which makes it amenable for electron paramagnetic resonance (EPR) studies (2-10). Such studies allow one to follow the changes in the electron distribution over the molecule associated with transitions between R and T states, considered to be of crucial importance in the explanation of cooperativity (11) in the oxygen-binding properties of hemoglobin. The second reason is that, in contrast to oxyhemoglobin and carbonmonoxyhemoglobin, NO-Hb shows a pronounced R-to-T transition (12,13) in the presence of inositol hexaphosphate, this transition being also rather sensitive to changes (5, 9, 10) in pH of the surrounding environment. One of the properties of NO-Hb that is a sensitive indicator of changes of structure under the influence of inositol hexaphosphate or changes in pH is the splitting produced (3-10) in EPR spectra by 14N hyperfine interactions. Thus, in NO-Hb A, in the presence of inositol hexaphosphate or at low pH one finds for the aY chain a 3-line hyperfine pattern associated with the hyperfine interaction of the '4N nucleus of NO. On increasing the pH beyond 8.0, even in the presence of inositol hexaphosphate one sees a change (5) from the 3-line to a 9-line pattern, indicating that th...
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