Wetlands are among the highly threatened ecosystems due to anthropogenic activities. The Ramaroshan Wetland Complex (RWC) of Achham District, Nepal is one of the high-altitude wetlands facing human induced degradation and loss. Herpetofauna are key bio-indicators of environmental health and habitat quality and are useful to assess habitat conditions of such threatened ecosystems. This study quantified the land use and land cover (LULC) change in the RWC and documented the diversity and distribution pattern of herpetofauna. The LULC in the area (13.94 Km2) was analyzed for 1989, 2000, 2010 and 2021 by supervised classification of remote sensing images. Surveys were conducted along 25 transects, each of 200 m in length and environmental variables were recorded for every observation of herpetofauna. The LULC analysis revealed an overall loss of 16% of the total water body between 1989 (0.25 Km2) and 2021 (0.21 Km2). Eleven species of herpetofauna (five amphibians and six reptiles) within five families and two orders (i.e., Anura and Squamata), were recorded with low diversity (H’ = 1.88312) and evenness (E = 0.3642) indices. The herpetofauna had a hump-shaped distribution along the elevation gradient with the highest richness and abundance at 2300 m asl. Amphibian abundance decreased with increasing distance to nearest water sources, whereas reptile abundance increased. Amphibians were more abundant in agricultural field and marsh land, whereas reptile abundance was higher around human settlements. Results indicate that the wetland area in the RWC is declining at an alarming rate and, in turn, might account for the low diversity and abundance of the herpetofauna.