The scaffolds and their interaction with mesenchymal stem cells are objects of study in bioengineering and tissue repair. Mechanisms such as surface adhesion, proliferation, viability, and cytotoxicity are essential for the development of therapies. The present study analyzed the influence of platelet-rich fibrin (PRF) in viability, cytotoxicity, and proliferation of stem cells from human exfoliated deciduous teeth (SHED) exposed to bovine biomaterial surfaces. The studied groups were divided and analyzed as follows: (S) only SHED as control Group; (SB) SHED + biomaterial; (SBP) SHED + biomaterial + PRF. Analyses of cells seeded in 24-well plates were performed after 24, 48 and 72 hours. Individual groups were subjected to viability, cytotoxicity and cell proliferation tests using neutral red, MTT and crystal violet, respectively; and in the 72-hour group, scanning electron microscopy (SEM) was performed to record cell ultra-morphology. Data were submitted to statistical analysis by two-factor ANOVA with a significance level of 5%. The results demonstrated a better performance in the viability/cytotoxicity and proliferation of stem cells for the group (SBP) in comparison to the group (SB) and the group (S). The applied statistical tests showed that the biomaterial factor, time, and interaction between them gave rise to results with statistical significance. SHED submitted to bovine biomaterial were more viable, proliferative and with lower toxicity when associated with PRF. PRF seemed to activate the metabolism of stem cells in culture, indicating that such an association can bring an effective benefit in clinical outcome.
Introdução: A esclerodermia é uma desordem pertencente a um grupo de doenças autoimunes do tecido conjuntivo, caracterizada por esclerose na pele, pulmões, coração, trato gastrointestinal, rins e sistema musculoesquelético. Das manifestações orais e periorais, são evidentes aumento do espaço do ligamento periodontal, limitação da função mastigatória, dificuldade no processo de reparo, fibrose da pele, diminuição da abertura bucal, xerostomia, cárie, entre outros.Em virtude das alterações na microcirculação dos tecidos gengivais e dos medicamentos sistêmicos, pode haver inflamação gengival e desenvolvimento de hiperplasia gengival. A xerostomia e as alterações escleróticas das mãos e dedos contribuem para um maior acúmulo de placa nesses pacientes. Objetivo: Relatar o caso clínico de uma paciente portadora de esclerodermia sistêmica (ES) e a associação dessa doença com as manifestações orais e periodontais. Relato decaso: Paciente A.M.S., 47 anos, sexo feminino, diagnosticada com ES há 16 anos, apresentou em associação à doença sistêmica diferentes manifestações orais e periodontais, como aumento do espaço do ligamento periodontal, diminuição da abertura bucal, limitação da função mastigatória, dificuldade no processo de reparo, fibrose da pele, xerostomia, cárie, sangramento gengival, presença de cálculo dental e mobilidade dentária. Conclusão: Os sinais e sintomas apresentados podem interferir na qualidade de vida dos pacientes. Portanto, é de extrema importância o cirurgião-dentista estar atento às manifestações, a fim de orientar e motivar os pacientes com o intuito de diminuir os fatores de risco, proporcionar um tratamento apropriado ou até mesmo realizar um possível diagnóstico da doença.
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