The low frequency array (LOFAR), is the first radio telescope designed with the capability to measure radio emission from cosmic-ray induced air showers in parallel with interferometric observations. In the first ∼2 years of observing, 405 cosmic-ray events in the energy range of 10 16 −10 18 eV have been detected in the band from 30−80 MHz. Each of these air showers is registered with up to ∼1000 independent antennas resulting in measurements of the radio emission with unprecedented detail. This article describes the dataset, as well as the analysis pipeline, and serves as a reference for future papers based on these data. All steps necessary to achieve a full reconstruction of the electric field at every antenna position are explained, including removal of radio frequency interference, correcting for the antenna response and identification of the pulsed signal.
Context. Low-frequency radio continuum observations (<300 MHz) can provide valuable information on the propagation of low-energy cosmic ray electrons (CRE). Nearby spiral galaxies have hardly been studied in this frequency range because of the technical challenges of low-frequency radio interferometry. This is now changing with the start of operations of LOFAR. Aims. We aim to study the propagation of low-energy CRE in the interarm regions and the extended disk of the nearly face-on spiral galaxy Messier 51. We also search for polarisation in M 51 and other extragalactic sources in the field. Methods. The grand-design spiral galaxy M 51 was observed with the LOFAR High Frequency Antennas (HBA) and imaged in total intensity and polarisation. This observation covered the frequencies between 115 MHz and 175 MHz with 244 subbands of 8 channels each, resulting in 1952 channels. This allowed us to use RM synthesis to search for polarisation. Results. We produced an image of total emission of M 51 at the mean frequency of 151 MHz with 20 resolution and 0.3 mJy rms noise, which is the most sensitive image of a galaxy at frequencies below 300 MHz so far. The integrated spectrum of total radio emission is described well by a power law, while flat spectral indices in the central region indicate thermal absorption. We observe that the disk extends out to 16 kpc and see a break in the radial profile near the optical radius of the disk. The radial scale lengths in the inner and outer disks are greater at 151 MHz, and the break is smoother at 151 MHz than those observed at 1.4 GHz. The arm-interarm contrast is lower at 151 MHz than at 1400 MHz, indicating propagation of CRE from spiral arms into interarm regions. The correlations between the images of radio emission at 151 MHz and 1400 MHz and the FIR emission at 70 μm reveal breaks on scales of 1.4 and 0.7 kpc, respectively. The total (equipartition) magnetic field strength decreases from about 28 μG in the central region to about 10 μG at 10 kpc radius. No significant polarisation was detected from M 51, owing to severe Faraday depolarisation. Six extragalactic sources are detected in polarisation in the M 51 field of 4.1 • × 4.1 • size. Two sources show complex structures in Faraday space. Conclusions. Our main results, the scale lengths of the inner and outer disks at 151 MHz and 1.4 GHz, arm-interarm contrast, and the break scales of the radio-FIR correlations, can be explained consistently by CRE diffusion, leading to a longer propagation length of CRE of lower energy. The distribution of CRE sources drops sharply at about 10 kpc radius, where the star formation rate also decreases sharply. We find evidence that thermal absorption is primarily caused by H ii regions. The non-detection of polarisation from M 51 at 151 MHz is consistent with the estimates of Faraday depolarisation. Future searches for polarised emission in this frequency range should concentrate on regions with low star formation rates.Key words. polarization -cosmic rays -galaxies: ISM -galaxies: ...
The actin system of highly motile cells runs far from equilibrium and generates a multitude of patterns within a dynamic filamentous network. Traveling waves are the most complicated patterns based on recruitment of the Arp2/3 complex. They are governed by the propagated induction of actin polymerization. We hypothesize that the actin system autonomously generates primordia of specialized structures such as phagocytic cups or lamellipodia. These primordia would represent an activated state of the actin system and enable cells to respond within seconds to local stimuli by chemotaxis or phagocytic-cup formation.
Cytokinesis in eukaryotic organisms is under the control of small GTP-binding proteins, although the underlying molecular mechanisms are not fully understood. Cortexillins are actin-binding proteins whose activity is crucial for cytokinesis in Dictyostelium. Here we show that the IQGAP-related and Rac1-binding protein DGAP1 specifically interacts with the C-terminal, actin-bundling domain of cortexillin I. Like cortexillin I, DGAP1 is enriched in the cortex of interphase cells and translocates to the cleavage furrow during cytokinesis. The activated form of the small GTPase Rac1A recruits DGAP1 into a quaternary complex with cortexillin I and II. In DGAP1(-) mutants, a complex can still be formed with a second IQGAP-related protein, GAPA. The simultaneous elimination of DGAP1 and GAPA, however, prevents complex formation and localization of the cortexillins to the cleavage furrow. This leads to a severe defect in cytokinesis, which is similar to that found in cortexillin I/II double-null mutants. Our observations define a novel and functionally significant signaling pathway that is required for cytokinesis.
The Arp2/3 complex is a ubiquitous and important regulator of the actin cytoskeleton. Here we identify this complex from Dictyostelium and investigate its dynamics in live cells. The predicted sequences of the subunits show a strong homology to the members of the mammalian complex, with the larger subunits generally better conserved than the smaller ones. In the highly motile cells of Dictyostelium, the Arp2/3 complex is rapidly re-distributed to the cytoskeleton in response to external stimuli. Fusions of Arp3 and p41-Arc with GFP reveal that in phagocytosis, macropinocytosis, and chemotaxis the complex is recruited within seconds to sites where actin polymerization is induced. In contrast, there is little or no localization to the cleavage furrow during cytokinesis. Rather the Arp2/3 complex is enriched in ruffles at the polar regions of mitotic cells, which suggests a role in actin polymerization in these ruffles.
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