As the hardest and one of the most durable load bearing tissues of the body, enamel has attracted considerable interest from both material scientists and clinical practitioners due to its excellent mechanical properties. The aim of this investigation is to determine the influence of different loading conditions on the deformation behavior of human enamel using instrumented indentation and Berkovich indenter. The used samples were fresh intact human premolars, extracted due to orthodontic reasons. Hardness tests were performed with different loading regimes. To study the influence of loading rates tree different loading rates have been used with 10, 100 and 1000 mN/min. at maximum applied load of 200 mN. The indentation size effect (ISE) was studied using loads from 5 mN to 400 mN without holding time. The indents have been studied using atomoc force microscopy and scanning electron microscopy.
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