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Mature sperm of Acheta domesticus, Acheta assimilis, Nemobius fasciatus, Nemobius confusus, Melanoplusfemur-rubrum, Romalea microptera, Scudderia curvicauda, and Ceuthophilus nigricans were examined for DNA configuration by means of polarized fluorescence microscopy. In all cases, the results suggest that the DNA lies in unsupercoiled array, predominantly parallel to the elongate sperm head axes. Detailed calculations of the factors influencing the degree of polarization of the fluorescent emission from supercoiled DNA are contained in an appendix.

10 Roguski, H., personal communication. 11 Evidence in support of this statement has been obtained by one of us (G. C.) in experiments in which only one of the first two blastomeres of o/o eggs is injected with normal cytoplasm. These embryos subsequently display correction of the maternal effect of gene o only in those regions derived from the injected blastomere.