This simplified procedure to detect aflatoxin in cottonseed and cottonseed products can be used at the field level where equipment for thin layer chromatography is not available. Contamination levels as low as 10 ppb can be detected in these columns or tubes using longwave uv light. Development and detection of aflatoxin in these tubes takes ca. 10 min.
Large samples called “sublots” were drawn from 41 commercial lots of contaminated cottonseed. Each sublot was subdivided into twenty 5 lb samples which were analyzed for aflatoxin. The mean, median, variance, coefficient of variation, and the estimated range among the sample concentrations were computed. The results indicated that: (A) the variance among sample concentrations was large and was found to be a function of sample concentration and (B) the distribution of sample concentrations was skewed; the density of sample values was greater below the sublot concentration.
Filter fluorometers have been adapted to measure the fluorescence intensity of aflatoxin adsorbed on a Florisil layer in minicolumns. The relationship between concentration and intensity is near linear in the aflatoxin range from 10 to 100 ng. Although individual aflatoxin fractions cannot be resolved, since the measure is one of total intensity, fluorometric measurements advance the minicolumn screening procedure to a semiquantitative level. The detection of 1 ng aflatoxin B1 is well within the limits of a filter fluorometer with a photomultiplier detector. A precision, expressed as percent coefficient of variation, ranging from 1.2 to 4.2%, was obtained for standard B1 columns.
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