An attempt has been made to investigate changes in the characteristics of aerosol optical properties induced during Diwali/ New Year celebrations of 2012 over an urban city, Ahmedabad (India). Ground based measurements of average Aerosol Optical Depth (AOD) were carried out using a Microtops-II at Physics Department, Gujarat University, Ahmedabad during 10 th to 16 th Nov, 2012. AOD on the day just after Diwali is found to increase significantly; around 75% compared to pre-Diwali days for visible wavelength, remained at higher level for one more day (next day of New Year). Turbidity factor (β) also changed considerably, to a high value of 0.34 on the next day of Diwali, indicating a significant increase in aerosol loading associated with Diwali/ New Year festivities. Normal anti-correlation between Angstrom exponent (α) and turbidity parameter (β) is not seen on days following Diwali. The AOD and β values in the evening of the next day of Diwali increased to about 164% and 171% respectively compared to pre-Diwali days evenings; such an increase associated with the festivities has not been reported at any part of the country. Spectral variation of AOD also shows a significant change in the pattern during following two days of celebrations compared to pre-Diwali days. It is seen to follow power law more closely. Significant increase in AOD and change in spectral pattern suggest an increase of fine/accumulation mode particles due to Diwali/ New Year festivities.
Mast cells are important effecter cells in allergic and inflammatory responses. When the mast cells are activated they undergo regulated secretion of intracellular granules by exocytosis. This includes inflammatory cytokines such as IL-6, TNF-α and other chemokines. In this study we examined mast cell activation using a microscope slide based pico-well array at a single cell resolution. The human umbilical cord blood derived mast cells were cultured in the mast cell medium. The cells were counted and 2uL of cell suspension were added to the pico-wells of the array (Livecell array, NUNC). The cells in the array were covered with a sliding cover slip allowing each cell to settle into an individual well. The cover slip also allowed for repeated treatment and washing of reagents via capillary flow. The cells were treated with substance P (SP) to induce activation directly on the array. Live cell imaging showed cells were distributed evenly on the array as one cell per well. After activation, the cells secreted granules which are clearly visible in the pico-wells surrounding the cells. Additionally, LAD2 cells were activated with compound 48/80 (0.1ug/ml). Use of anti-CD63 PE permitted single cell investigation of degranulation. The ability to culture, treat and image non-adherent mast cells in the array allowed us to study single cell responses in a mast cell population, and permits fast screening of potential inhibitors.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.