Recent experiments support the idea that upper thermal limits of aquatic insects arise, at least in part, from a lack of sufficient oxygen: rising temperatures typically stimulate metabolic demand for oxygen more than they increase rates of oxygen supply from the environment. Consequently, factors influencing oxygen supply, like water flow, should also affect thermal and hypoxia tolerance. We tested this hypothesis by measuring the effects of experimentally manipulated flows on the heat and hypoxia tolerance of aquatic nymphs of the giant salmonfly (Plecoptera: Pteronarcys californica ), a common stonefly in western North America. As predicted, stoneflies in flowing water (10 cm s −1 ) tolerated water that was approximately 4°C warmer and that contained approximately 15% less oxygen than did those in standing water. Our results imply that the impacts of climate change on streamflow, such as changes in patterns of precipitation and decreased snowpack, will magnify the threats to aquatic insects from warmer water temperatures and lower oxygen levels.

Like all taxa, populations of aquatic insects may respond to climate change by evolving new physiologies or behaviors, shifting their ranges, exhibiting physiological and behavioral plasticity, or by going extinct. We evaluated the importance of plasticity by measuring changes in growth, survival, and respiratory phenotypes of salmonfly nymphs (the stonefly Pteronarcys californica) in response to experimental combinations of dissolved oxygen and temperature. Overall, smaller individuals grew more rapidly during the six-week experimental period, and oxygen and temperature interacted to affect growth in complex ways. Survival was lower for the warm treatment, though only four mortalities occurred (91.6 vs 100%). Nymphs acclimated to warmer temperatures did not have higher critical thermal maxima (CTMAX), but those acclimated to hypoxia had CTMAX values (in normoxia) higher by approximately 1 °C. These results suggest possible adaptive plasticity of systems for taking up or delivering oxygen. We examined these possibilities by measuring the oxygen-sensitivity of metabolic rates and the morphologies of tracheal gill tufts located ventrally on thoracic and abdominal segments. Mass-specific metabolic rates of individuals acclimated to warmer temperatures were higher in acute hypoxia but lower in normoxia, regardless of their recent history of oxygen exposure during acclimation. The morphology of gill filaments, however, changed in ways that appeared to depress rates of oxygen delivery in functional hypoxia. Our combined results from multiple performance metrics indicate that rising temperatures and hypoxia may interact to magnify the risks to aquatic insects, but that physiological plasticity in respiratory phenotypes may offset some of these risks.

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