When [ 1 -14C]palmitic acid was incubated with young pea leaves or solutions of their acetonedried powders, 2-hydroxy [l-14C]palmitic acid accumulated. This metabolite has been isolated as its methyl ester. When diluted with unlabelled synthetic methyl D-2-hydroxypalmitate to known specific activity, and recrystallised, the crystals and mother liquor residues retained the same specific activity. When diluted with unlabelled synthetic methyl L-2-hydroxypalmitate to known specific activity and recrystallised, the crystals were almost non-radioactive. The radioactive metabolite therefore has the D-COnfigUratiOn.Unlabelled 2-hydroxypalmitic acid isolated from pea leaves was shown to have the n-configuration by direct measurement.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.