It is usually assumed that enzymes retain their native structure during catalysis. However, the aggregation and fragmentation of proteins can be difficult to detect and sometimes conclusions are drawn based...
BaTiO 3 is widely used as the dielectric in ceramic chip capacitors and multilayer capacitors, because of its high dielectric constant and ferroelectric properties. Multilayer capacitors provide fairly high capacitance per unit volume (volumetric efficiency); however, processing difficulties in the preparation of ultrathin layers limit further enhancement. Tantalum solid electrolytic capacitors, on the other hand, provide very high volumetric efficiencies, because of the large surface area of the sintered, porous tantalum anode on which the dielectric Ta 2 O 5 is electrochemically deposited. Recent developments in electrochemical methods to deposit BaTiO 3 on titanium substrates provide an opportunity to fabricate barium titanate electrolytic capacitors using sintered, porous titanium anodes. The high dielectric constant of BaTiO 3 and the high surface area of the sintered, porous anode provide a good combination to achieve larger volumetric efficiencies. Current work involves the fabrication and characterization of barium titanate electrolytic capacitors. Effects of electrochemical processing parameters on the formation of BaTiO 3 on the surface of sintered titanium anodes are described. Influence of the purity of titanium powder, the porosity of the sintered anode, and the post-deposition heat treatment on the dielectric properties of the fabricated capacitors is discussed. Complete penetration of the electrolyte solution and a thin uniform coating of TaTiO 3 over the entire titanium surface was achieved using high-porosity (35%-40% of theoretical density) sintered titanium anodes. Samples treated for 8 h in 0.5M Ba(OH) 2 ؒ8H 2 O electrolyte solutions at 100°C with an applied cell voltage of 12 V show the formation of a dense, uniform BaTiO 3 coating on the surface of the titanium anode. High-purity, chloride-free titanium powder provides smaller dissipation factors at low frequencies. Heat treatment at 400°C significantly increases the capacitance at all frequencies, whereas the heat treatment lowers the dissipation factors at low frequencies. Calculated volumetric efficiencies are comparable to those typically obtained for tantalum solid electrolytic capacitors but are not as high as expected for barium titanate electrolytic capacitors. Penetration of the colloidal-carbon (external) electrode was limited to a depth of ∼300 µm, which might have caused the lower volumetric efficiencies.
It is usually assumed that enzymes retain their native structure during catalysis. However, the aggregation and fragmentation of proteins can be difficult to detect and sometimes conclusions are drawn based on the assumption that the protein is in its native form. We have examined three model enzymes, alkaline phosphatase (AkP), hexokinase (HK) and glucose oxidase (GOx). We find that these enzymes aggregate or fragment after addition of chemical species directly related to their catalysis. We used several independent techniques to study this behavior. Specifically, we found that glucose oxidase and hexokinase fragment in the presence of D-Glucose but not L-glucose, while hexokinase aggregates in the presence of Mg2+ ion and either ATP or ADP at low pH. Alkaline phosphatase aggregates in the presence of Zn2+ ion and inorganic phosphate. The aggregation of hexokinase and alkaline phosphatase does not appear to attenuate their catalytic activity. Our study indicates that specific multimeric structures of native enzymes may not be retained during catalysis and suggests pathways for different enzymes to associate or separate over the course of substrate turnover.
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