Center, Los Angeles, CA 90009. It was reviewed and approved for The Aerospace Corporation by W. R. Warren, Jr., Director, Aerophysics Laboratory. Lieutenant James C. Garcia, SD/YLXT, was the project officer for Technology. This report has been reviewed by the Public Affairs Office (PAS) and is releasable to the National Technical Information Service (NTIS). At NTIS, it will be available to the general public, including foreign nations. This-.hnical report as been reviewed and is approved for publication. PublicationoTlf.ilTreport does not constitute Air Force approval of the report's findings or conclusions. It is published only for the exchange and stimulation of ideas.
Human tissues are composed of complex admixtures of diVerent cell types and their biologically meaningful analysis necessitates the procurement of pure samples of the cells of interest. Many approaches have been used in attempts to overcome this diYculty, including a variety of microdissection methods. This review concerns a recent advance in microdissection techniques, namely laser capture microdissection (LCM). The principle underlying this technique is outlined, and practical issues pertaining to LCM are considered. In addition, the literature relating to LCM is reviewed, with examples of research applications of this technique being outlined. (J Clin Pathol: Mol Pathol 2000;53:64-68)
It has been possible to obtain mirrors of very high reflectivity by following the simple four-step procedure described herein. The key to success is the ability to measure the scattering and other losses of the substrates and dielectric coatings to ensure that the specifications are being met. These measurements are especially critical in the important cleaning process. The cavity-attenuated phase-shift (CAPS) method is ideally suited for performing these important measurements, permitting us to obtain mirrors with reflectivities of R = 0.99975 +/- 0.00005.
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