In a previous publication the writers (38) have described their experiments to determine the influence of environal factors on the infection of sorghums by covered and loose kernel smuts. The present paper takes up a similar series of experiments on the infection of oats by Ustilago levis (K. & S.) Magn. The methods employed were essentially the same as described for the previous studies.In 1922 a preliminary test was made to determine the influence of moisture on the infection of Avena nuda L. var. inermis (S. N. 30) by both Ustilago avenae (Pers.) lens. and U. levis (K. & S.) Magn. The experiment was planned primarily to determine whether sand would be a suitable substratum for carrying out an extended series of experiments on the influence of temperature, moisture, and other factors on infection. The sand used passed through a zo-mesh sieve but not through a 4o-mesh sieve, and had a reaction of pH 7.2. The seeds were germinated in the laboratory at a temperature of 19°-22°C. Four soil moistures were used-20, 40, 60, and 80 percent. The results are given in table I. TABLE I. Influence of Moisture on Infection of Avena nuda L. var. inermis (S. N.30) by Ustilago avenae and U. levis, I922; Laboratory Temperature, Sand, pH 7.2
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