Herpes simplex virus (HSV) is one of the common pathogenic viruses of humans bing. This study aimed to determin anti-herpes virus activity of Mentha Piperita extracts in vitro. Mentha Piperita extracts can inhibit HSV infection when the cells treated before viral adsorbtion. HSV-1 were also inhibited by menthol after viral adsorption. HSV-1 viral particles were directly inhibited and viral yield was reduced when treated with Mentha Piperita extracts and menthol. Therefore, Mentha Piperita extracts and menthol showed anti-HSV activities at various stages of the viral replication cycle.
Background and Aims: Cytomegalovirus (CMV) infection is very common in the population. Virus belongs to the family Herpesviridae, whose representatives are characterized by the ability to cause the human body's latent persistent infections. The goal of the study is to assess the CMVV infection frequency and PCR method in the choice of CMV in inflammatory eye disease, Comparing with CMV presence in Eye Infection and the control group. Materials and Methods: Primers were designed for conserved regions of the CMV genome. We have used PCR to rapid, accurate detection of EBV DNA in blood and from eye swabs as well as pp65 antigenemia. We have chosen to study patients with eye inflammation or infection symptom. Results: CMV DNA was detectable in three 21 out of 130 control samples of serum (16.5%). CMV infection was seen in 9 out of 20 (45%) patients' serum samples. Compared with the controls, the presence of EBV DNA was only significantly increased in samples of the patient group. 13 out of 20 (65%) patients in patients and 19 out of 130 (14.61%) of the control group had detectable CMV DNA in their ocular swab. Conclusion: We have presented evidence for the presence of CMV sequences in normal and eye inflammation samples with PCR. CMV serology was available for a large number of individuals. The prevalence of CMV in ocular disease samples varied dramatically that this wide range may be due to variations and inconsistency in the techniques used to detect the virus and its Components, as well as geography and genetic susceptibility.
Cytomegalovirus is the most important pathogen affecting transplant recipients and causing significant morbidity and mortality. The prevalence of CMV infections in transplant recipient varies from country to country. The aim of this study was to determine the prognostic value of PCR assay for CMV detection in patients before Keratoplasty. A total of 23 patient samples enrolled in this study from March 2008 to Feb 2010. Based on the result the over all incidence of CMV infection was 39.1 percent (9 positive sample) in PCR assay. In this study, the plasma PCR assay proved to be sensitive and specific in order to detect CMV infections.
Background and Aims: Herpes simplex virus (HSV) is a common cause of corneal disease and is the leading infectious cause of corneal blindness among developed nations. This study is aimed to provide an estimation of the incidence of Herpes Simplex Virustype-1, 2(HSV1, 2) and Varicella Zoster Virus (VZV) in tears and swab from eye infection by polymerase chain reaction (PCR) in eye disease in a suspected community. Materials and Methods: Fifty subjects without signs of ocular herpetic disease enrolled in the study. Serum samples from all subjects were tested for HSV IgG antibodies by enzymelinked immunosorbent assay (ELISA). Subjects were instructed to collect tear samples by touching the inner surface of the lower eyelid with an individually wrapped, sterile cotton swab and to place the swab immediately into labeled sterile tubes. Swabs were kept at 4°C until processed. Nucleic acid was extracted from the samples and PCR-amplified for HSV DNA. Results: Among 50 samples, 3 samples were refused because internal controls were negative. HSV infection was established in 10% (5 out of 50) of all patients. The prevalence of HSV infection in patients with no clinical suspicion of herpetic keratitis was 6%. Histopathologic evaluation revealed that among samples with positive PCR results, 100% had evidence of inflammation, 55% had giant cells, 39% had necrosis, 59% had vascularization, 67 % had ulcer and 100% of them had inclusion bodies. Conclusions: Because some of the patients with no clinical suspicion of herpes infection were found positive, we suggest that HSV to be considered as one of the underlying etiologies in any patient with corneal scar and keratitis. Therefore, performing further diagnostic methods, including PCR and histopathology, is mandatory to clearly understand the infection.
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