Bacteriocins are bacterial antimicrobial peptides with bactericidal activity against other bacteria. Staphylococcins are bacteriocins produced by staphylococci, which are gram-positive bacteria with medical and veterinary importance. In this study we are using two promoters from two plasmids to lysostaphin gene to change production of lysostaphin protein then killed Staphylococcus aureus and compared with pBluescript plasmid as control, pTrc99a get increased the production of lysostaphin protein while the pBAD30 are decreased the production in compared with pBluescript plasmid as control, at the same time both plasmid reported succeeded to lysostaphin expression and gave the protein with different concentration.

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Lysostaphin is a protein zinc metalloproteinase, extracted from Staphylococcus simulans, which disrupting peptide layer of S. aureus. In this study, Lysostaphin gene was detected in the S. simulans isolates. The molecular weight of the Lysostaphin gene is 750 bp. We were used the pET-32a(+) plasmid to cloning lysostaphin gene which transformed to competent rubidium chloride E. coli DH5α for producing the lysostaphin protein. The lysostaphin protein from this gene which isolated from S. simulans, then used the expression of used to killed S. aureus, which has the thick layer of wall that is the very difficult bacteria response to treatment. The result was reported succeeded pET-32a (+) plasmid to expressed lysostaphin gene and gave lysostaphin protein with high quality and quantity. As well as the result was appeared the high accuracy of his tag method in protein extraction and purification, and the quality and quantity more than other studies.

Hydatid disease or Echinoccosis is one of the serious public health problems. This study was designed as a comparative molecular study between some plant extract and tinidazole of sheep hydatid cysts in Basra province. In this study, 213 sheep were examined and the number of those infected with hydatid cysts was 75 (35.2 %). The results showed that hydatid cyst was detected successfully by performing PCR technique. In addition, Quercus aegilops has been shown the best plant extract for destroying all genes, the second plant extract which is Capparis spinosa showed destroying of two genes (G6-7, COI) and failed to destroy (sh4-1). Furthermore, Prosopis fracta has shown to destroy only (G6-7) and tinidazole failed to destroy any of these genes used in this study.

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Hydatid disease or Echinoccosis is one of the serious public health problems. This study designed for molecular investigation of Hydatid cysts in different hosts animals (Sheep, donkey) and human in Basra city, as well as molecular characterization by PCR technique to determine the specific gene for human (ND1, COI, CO1), sheep (G6-7, sh4-1, COI) and donkey (COI, CO1). Then, gene sequencing to recognize the intraspecific variation of Echincoccus in relation to host. In the current study, 818 sheep were examined and the number of sheep infected with hydatid cysts was 271. Donkey samples (10) were brought to the animal field of Veterinary Medicine at the University of Basra and the number of infected with hydatid cysts was two in the period from September 2017 to March 2018. The hydatid cysts were collected from the human after surgery at Al-Sadr Teaching Hospital where 21. The results were higher in female (15) than male (6) in the same period.