Summary:The tolerance to degradation processes in meat of nine Trichinella genotypes was studied in mouse and fox tissue, respectively. Minced muscle tissue with Trichinella larvae of different age was stored at room temperature at 100 % relative humidity. During storage weekly sub samples of the minced meat were digested and released larvae were inoculated in mice to evaluate the Reproductive Capacity Index (RCI). The RCI decreased with the length of storage, but the larvae from older infections appeared better adapted to tolerate the degradation processes. The African species T. nelsoni had a relative higher tolerance to elevated temperature during storage and the unencysted species T. pseudospiralis was the most vulnerable genotype. KEY WORDS :Trichinella, muscl e tissue, foxes, mice, decaying meat, degradation, reproducti ve capaci ty. T he nematodes in the genusTrichinella has evolved an amazing and complex protection system of interaction with the host muscle cell.The formation of a cyst around the larvae, the so called "nurse cell", with a layer of collagen on the outside protects and maintains the parasite. A set of blood vessels beneath the collagen capsule, the circulatory rete, provides the muscle larvae with nutrients (Despommier, 1998).The scavenging nature of many Trichinella hosts favour the ability of the muscle larvae to survive in the muscle tissue after the death of the host. The carcass will not lay for a long time before the first animals will scavenge on it. Even so the parasite has to be able to withstand to some extent the cadaveric poisons in the meat, in order to survive transmission to a new host. 1979; Boev et al., 1979) and differences between the known genotypes. The aim of the present study was to evaluate how long the parasite can survive and withstand the degradation processes in the dead meat.Furthermore genotypic characteristics were studied to identify differences between isolates from the northern hemisphere and tropical isolates. to six weeks of storage the muscle larvae (ML) were recovered from the meat samples by digestion according to Gamble (1996). A control sample (day 0) was digested immediately after mincing. The reproductive capacity index (RCI = recovered larvae/ inoculated larvae) of the recovered larvae was evaluated in a maximum of four mice for each sample. An inoculation dose of 500 larvae/mouse was used. If less than 500 larvae were recovered from the rotten meat, only one mouse were inoculated. Storage room temperature were at 22° C ± 1, besides from slightly elevated temperatures in the summer time (27(C (2 in five and ten weeks old infections). MATERIALS AND METHODS
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