Reproductive strategies can act as strong selective forces on reproductive traits of male insects, resulting in species-specific variation in sperm quantity and viability. For solitary bees, basic measures of sperm quantity and viability are scarce. Here we evaluated for the first time quantity and viability of sperm in male Osmia cornuta solitary bees at different times after emergence, and how they were affected by male body mass and environmental condition (laboratory or semi-field arena). Sperm viability immediately after adult emergence showed no significant difference compared to four day old individuals, suggesting that O . cornuta males are capable of mating immediately post emergence. However, sperm counts were significantly higher in four day old individuals from the semi-field arena when compared to newly emerged males. This might reflect a final phase of sperm maturation. Regardless of individual male age and body mass differences, O . cornuta males produced on average ~175’000 spermatozoa that were ~65% viable, which are both significantly lower compared to eusocial honeybees and bumblebees. Moreover, sperm quantity, but not viability, was positively correlated with male body mass four days after emergence, while no such relationship was detected immediately after emergence. Even though individuals maintained in semi-field conditions exhibited a significantly greater loss of body mass, experimental arena had no significant effect on male survival, sperm quality or total living sperm produced. This suggests that the proposed laboratory design provides a cost-efficient and simple experimental approach to assess sperm traits in solitary bees. In conclusion, our data suggest a reduced investment in both sperm quantity and quality by male O . cornuta , which appears to be adaptive in light of the life history of this solitary bee.
This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Bee venom (BV), or apitoxin, is a complex substance produced by a gland in the abdominal cavity of bees. The main component of BV is melittin, which is a largely studied substance due to its biological properties. To date, the most well-known bee venom and melittin are derived from domesticated honey bees, while venom and melittin derived from wild honey bees have been under-investigated. Hence, this study primarily reports the antimicrobial activities of bee venom and synthetic melittin derived from four different honey bee species (Apis mellifera, A. cerana, A. dorsata, and A. florea) in Thailand. All the bee venom extracts and melittins showed more robust antibacterial activities against Gram-positive (Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, S. aureus MRSA, and S. epidermidis) than Gram-negative bacteria (Escherichia coli, Klebsiella pneuminiae, and Salmonella typhimurium) or a fungus (Candida albicans), while the synthetic melittins also have antimicrobial activity at higher concentrations than the bee venom extract. Furthermore, the A. cerana venom extract showed the highest activity against the tested bacteria, followed by A. mellifera, A. florea, and A. dorsata. Therefore, A. cerana venom may be further developed for use in medical applications as a potential alternative agent against Gram-positive bacteria and antibiotic-resistant bacteria.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.