Highly specific antibodies to 13, 14-di hydro\x=req-\ 15-ketoprostaglandin F2\g=a\(PGFM) were raised in rabbits.The animals were immunized with PGFM-bovine serum albumin (BSA)-conjugates. Prior to the incubation procedure PGFM was extracted by a rapid method with dichloromethane followed by column chromatography.The antisera dilution was 1:10000 and the cross-reactivity towards prostaglandin A2, E2, F2\g=a\,13, 14-dihydro\x=req-\ 15-ketoprostaglandin E2 and the 15-ketoprostaglandin E2 and F2\g=a\ was < 1%. The limit of detection was 1.9 \ m=+-\ 0.6 pg/ml plasma over the standard range 1.9\p=n-\250 pg. The intra-and inter-assay variations were 3.9 and 15%, respectively. PGFM was measured throughout the menstrual cycle in female volunteers.In normal ovulatory women (n = 3) plasma levels of PGFM varied between 65.6 to 107.1 pg/ml. No significant variations of plasma PGFM were seen during the cycle. In anovulatory women (n = 4) no difference of PGFM was found during the cycle. PGFM levels in hyperprolactinaemic but ovulating women tend to be higher than in anovulatory, and normoprolactinaemic subjects. These data strongly indicate that PGFM is not correlated with other hormonal parameters tested here in the normal and anovulatory cycles.
Se realizó un estudio abierto, prospectivo y multicéntrico en Colombia con el objeto de evaluar la eficacia, la seguridad anticonceptiva, el control del ciclo y los efectos secundarios de la nueva píldora anticonceptiva con 75 µg de gestodeno y 30 f.ug de etinilestradiol durante 12 ciclos consecutivos.Se reclutaron 186 mujeres. Se analizan 168 ciclos de 72 mujeres quienes completaron el período de observación de 12 ciclos. Se documentaron un total de 1.358 ciclos.El control del ciclo menstrual fue adecuado. La duración del mismo, así como la intensidad y la duración de la hemorragia se redujeron ligeramente, permaneciendo dentro de los límites de la normalidad. No se presentaron alteraciones clínicas de importancia como tromboembolismo o trastornos hepáticos. Los cambios en el peso corporal y en los valores de la presión sanguínea no fueron significativos. Las tasas de efectos secundarios y de abandono fueron similares a aquellas informadas para otras píldoras de microdosis. Se obtuvo un índice de Pearl de 0.12, lo cual confirma la eficacia y la seguridad anticonceptiva, incluso en los ciclos con olvido de grageas (3.8%). Los estudios epidemiológicos a largo plazo permitirán validar la bondad de esta nueva píldora.
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