No abstract

Bolete Productivity of Cistaceous Scrublands in Northwestern Spain. Cistaceous scrublands dominated by Cistus ladanifer and Halimium lasianthum are widely distributed in northwestern Spain and are known to harbor the popular edible mushrooms Boletus edulis and B. aereus. As commercial harvest of these species is an important activity in Spain's forests, in some cases generating greater revenue than timber production, we tried to determine whether there is commercial potential for bolete harvest in young cistaceous scrublands. Transects of 50 m × 2 m were randomly established in cistaceous scrublands and bolete yield was measured. High production of these valuable mushrooms was associated with 3-year-old plants, a much earlier onset than observed for Pinus and Quercus stands. Management recommendations for the studied scrub ecosystems are proposed that take into account the economic value of the mushrooms.Producción de Boletus asociados a matorrales de Cistaceas en el Noroeste de España. Grandes masas de Cistaceas dominadas por Cistus ladanifer and Halimium lasianthum están ampliamente distibuidas por el Noroeste de la península ibérica y es posible encontrar hongos apreciados como Boletus edulis y B. aereus. Nosotros intentamos determinar el potencial comercial del aprovechamiento de estas especies en matorrales jóvenes ya que en muchas zonas puede suponer una actividad económica que supere los beneficios procedentes de la madera. La producción de Boletus se recogió en parcelas de 2×50 metros. Se pudo observar una alta producción de estas especies asociada a plantas de 3 años, una edad mucho más temprana que la necesaria en masas de Pinus o Quercus. Finalmente, se proponen alternativas de gestión para estas particulares zonas teniendo en cuenta el valor económico de los hongos.

No abstract

Boletus edulis Bull. is one of the most economically and gastronomically valuable fungi worldwide. Sporocarp production normally occurs when symbiotically associated with a number of tree species in stands over 40 years old, but it has also been reported in 3-year-old Cistus ladanifer L. shrubs. Efforts toward the domestication of B. edulis have thus focused on successfully generating C. ladanifer seedlings associated with B. edulis under controlled conditions. Microorganisms have an important role mediating mycorrhizal symbiosis, such as some bacteria species which enhance mycorrhiza formation (mycorrhiza helper bacteria). Thus, in this study, we explored the effect that mycorrhiza helper bacteria have on the efficiency and intensity of the ectomycorrhizal symbiosis between C. ladanifer and B. edulis. The aim of this work was to optimize an in vitro protocol for the mycorrhizal synthesis of B. edulis with C. ladanifer by testing the effects of fungal culture time and coinoculation with the helper bacteria Pseudomonas fluorescens Migula. The results confirmed successful mycorrhizal synthesis between C. ladanifer and B. edulis. Coinoculation of B. edulis with P. fluorescens doubled within-plant mycorrhization levels although it did not result in an increased number of seedlings colonized with B. edulis mycorrhizae. B. edulis mycelium culture time also increased mycorrhization levels but not the presence of mycorrhizae. These findings bring us closer to controlled B. edulis sporocarp production in plantations.

No abstract

Long-term field-data series were used to fit a mushroom productivity model. Simulations enabled us to predict the consequences of management and climate scenarios on potential mushroom productivity. Mushrooms play an important ecological and economic role in forest ecosystems. Human interest in collecting mushrooms for self-consumption is also increasing, giving forests added value for providing recreational services. Pinus pinaster Ait. is a western Mediterranean species of great economic and ecological value. Over 7.5% of the total European distribution of the species is found on the Castilian Plateau in central Spain, where a great variety of mushrooms can be harvested. The aim of this study was to model and simulate mushroom productivity in Maritime pine (Pinus pinaster Ait.) ecosystems in northern Spain under different silvicultural and climatic scenarios. A mixed model was fitted that related total mushroom productivity to stand and weather variables. The model was uploaded to the SiManFor platform to study the effect of different silvicultural and climatic scenarios on mushroom productivity. The selected independent variables in the model were the ratio between stand basal area and density as a stand management indicator, along with precipitation and average temperatures for September and November. The simulation results also showed that silviculture had a positive impact on mushroom productivity, which was higher in scenarios with moderate and high thinning intensities. The impact was highly positive in wetter scenarios, though only slightly positive and negative responses were observed in hotter and drier scenarios, respectively. Silviculture had a positive impact on mushroom productivity, especially in wetter scenarios. Precipitation had greater influence than temperature on total mushroom productivity in Maritime pine stands. The results of this paper will enable forest managers to develop optimal management approaches for P. pinaster forests that integrate Non-Wood Forest Products resources.

No abstract

Summary Cistus ladanifer scrublands, traditionally considered as unproductive, have nonetheless been observed to produce large quantities of king bolete (Boletus edulis) fruitbodies. These pyrophytic scrublands are prone to wildfires, which severely affect fungi, hence the need for fire prevention in producing C. ladanifer scrublands. In addition, B. edulis productions have severely decreased in the last years. A deeper understanding of the B. edulis life cycle and of biotic and abiotic factors influencing sporocarp formation is needed to implement management practices that facilitate B. edulis production. For example, some bacteria likely are involved in sporocarp production, representing a key part in the triple symbiosis (plant–fungus–bacteria). In this study, we used soil DNA metabarcoding in C. ladanifer scrublands to (i) assess the effect of site history and fire prevention treatment on bacterial richness and community composition; (ii) test if there was any correlation between various taxonomic groups of bacteria and mycelial biomass and sporocarp production of B. edulis; and to (iii) identify indicator bacteria associated with the most productive B. edulis sites. Our results show that site history drives bacterial richness and community composition, while fire prevention treatments have a weaker, but still detectable effect, particularly in the senescent plots. Sporocarp production correlated positively with genera in Verrucomicrobia. Several genera, e.g. Azospirillum and Gemmatimonas, were identified as indicators of the most productive sites, suggesting a potential biological role in B. edulis fructification. This study provides a better understanding of the triple symbiosis (plant–fungus–bacteria) involved in C. ladanifer–B. edulis systems.