Methods for the detection of Alicyclobacillus acidoterrestris at a level of 1 cell per 100 ml and enumeration with a sensitivity of 5 cells ml−1 were developed. Spores of A. acidoterrestris survived pasteurization and outgrew and multiplied at a similar rate to vegetative cells in both orange juice and apple juice. Alicyclobacillus acidoterrestris grew readily in orange juice, apple juice and a non‐carbonated fruit juice‐containing drink at temperatures of 25–44°C producing a taint and elevated levels (1–100 ppb) of guaiacol. Isolates of A. acidoterrestris can be identified using the DuPont RiboPrinter. It was isolated from apple drinks, apple juice concentrate and freshly squeezed orange juice.

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After oxidising sulphides to sulphates with a mixture of sodium chlorate and hydrochloric acid, the sample is refluxed with a reducing mixture of sodium iodide, red phosphorus, hypophosphorous acid, orthophosphoric acid and propionic acid. The hydrogen sulphide generated is absorbed in potassium hydroxide solution and titrated against 2-(hydroxymercuri)benzoic acid solution with dithizone as indicator. The approximate range covered is 5 t o 2000 mg kg-1 of sulphur in the rock.

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A simple method for determining parasorbic acid (I) in sorbic acid (II), suitable for usedown to levels of 0.5 mg/kg, is described. The parasorbic acid is extracted from aqueous potassium sorbate with dichloromethane and determined by gas chromatography using a flame ionisation detector.