Three strains of a butyrate-producing bacterium were isolated from the rumen contents of grazing sheep and cows. The strains were anaerobic, with Gram-positive cell walls, straight-to-slightly-curved, rod-shaped, non-spore-forming and single flagellate. C, C, C and C were the predominant fatty acids. The cell-wall peptidoglycan type was A1. The DNA G+C content varied from 41.4 to 42.2 mol%. 16S rRNA gene sequence similarities between the isolates and , and were found to be 96, 95 and 95 %, respectively. The phylogenetic tree showed that the strains constituted a different taxon, separate from other taxa with validly published names and forming a cluster with strains of On the basis of phenotypic, chemotaxonomic and phylogenetic results (16S RNA, ,, genes), the isolates are considered to represent a novel species of a new genus of the family, for which the name gen. nov., sp. nov. is proposed (type strain JK623 = DSM 29029 = LMG 28559). We also propose the transfer of to the new genus gen. nov., comb nov. This new genus represents saccharoclastic, chemo-organotrophic and obligatory anaerobic, non-spore-forming rods with Gram-positive membrane. The main fermentation products on peptone yeast glucose (PYG) medium were butyrate, acetate, hydrogen and lactate. The type species of the genus is gen. nov., comb nov. (Prévot, 1938) with type strain ATCC 33656 ( = JCM 17463).
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