Summary
Keratin degradation is of great interest for converting agro‐industrial waste into bioactive peptides and is directly relevant for understanding the pathogenesis of superficial infections caused by dermatophytes. However, the mechanism of this process remains unclear. Here, we obtained the complete genome sequence of a feather‐degrading, extremely thermophilic bacterium, Fervidobacterium islandicum AW‐1 and performed bioinformatics‐based functional annotation. Reverse transcription PCR revealed that 57 putative protease‐encoding genes were differentially expressed in substrate‐dependent manners. Consequently, 16 candidate genes were highly expressed under starvation conditions, when keratin degradation begun. Subsequently, the dynamic expression profiles of these 16 selected genes in response to feathers, as determined via quantitative real‐time PCR, suggested that they included four metalloproteases and two peptidases including an ATP‐dependent serine protease, all of which might act as key players in feather decomposition. Furthermore, in vitro keratinolytic assays supported the notion that recombinant enzymes enhanced the decomposition of feathers in the presence of cell extracts. Therefore, our genome‐based systematic and dynamic expression profiling demonstrated that these identified metalloproteases together with two additional peptidases might be primarily associated with the decomposition of native feathers, suggesting that keratin degradation can be achieved via non‐canonical catalysis of several membrane‐associated metalloproteases in cooperation with cytosolic proteases.
Infestation of phosphine (PH3) resistant insects threatens global grain reserves. PH3 fumigation controls rice weevil (Sitophilus oryzae) but not highly resistant insect pests. Here, we investigated naturally occurring strains of S. oryzae that were moderately resistant (MR), strongly resistant (SR), or susceptible (wild-type; WT) to PH3 using global proteome analysis and mitochondrial DNA sequencing. Both PH3 resistant (PH3–R) strains exhibited higher susceptibility to ethyl formate-mediated inhibition of cytochrome c oxidase than the WT strain, whereas the disinfectant PH3 concentration time of the SR strain was much longer than that of the MR strain. Unlike the MR strain, which showed altered expression levels of genes encoding metabolic enzymes involved in catabolic pathways that minimize metabolic burden, the SR strain showed changes in the mitochondrial respiratory chain. Our results suggest that the acquisition of strong PH3 resistance necessitates the avoidance of oxidative phosphorylation through the accumulation of a few non-synonymous mutations in mitochondrial genes encoding complex I subunits as well as nuclear genes encoding dihydrolipoamide dehydrogenase, concomitant with metabolic reprogramming, a recognized hallmark of cancer metabolism. Taken together, our data suggest that reprogrammed metabolism represents a survival strategy of SR insect pests for the compensation of minimized energy transduction under anoxic conditions. Therefore, understanding the resistance mechanism of PH3–R strains will support the development of new strategies to control insect pests.
Finding potential electrodes with smart construction and design is one of the major tasks in the development of advanced sodium-ion batteries (SIBs) and in bridging the gap by bringing them to realization. In this work, we report on the design of such a binder-free composite anode realized by combining vertical carbon nanotube/molybdenum sulfide (VCNT/MoS 2 ) hybrid nanostructures. The well-aligned vertical nanostructures in the binder-free VCNT/MoS 2 hybrid composite electrode enhance the electrolyte penetration into the electrode and shorten the ion diffusion channels. Moreover, the VCNT provides better ion/ electron conductivity for the electrode. Due to these favorable characteristics, binder-free VCNT/MoS 2 hybrid composite electrodes exhibit superior electrochemical properties when employed as an anode for SIBs, especially superior rate capability with a specific discharge capacity of 403 mA h g −1 at a high current density of 3200 mA g −1 . The findings are expected to lead us in the direction of designing smart electrodes with hybrid nanostructured active materials, completely free of any binder for high-performing electrochemical energy storage applications.
Excavating the molecular details of many diverse enzymes from metagenomes remains challenging in agriculture, food, health, and environmental fields. We present a versatile method that accelerates metabolic enzyme discovery for highly selective gene capture in metagenomes using next‐generation sequencing. Culture‐independent enzyme mining of environmental DNA is based on a set of short identifying degenerate sequences specific for a wide range of enzyme superfamilies, followed by multiplexed DNA barcode sequencing. A strategy of ‘focused identification of next‐generation sequencing‐based definitive enzyme research’ enabled us to generate targeted enzyme datasets from metagenomes, resulting in minimal hands‐on obtention of high‐throughput biological diversity and potential function profiles, without being time‐consuming. This method also provided a targeted inventory of predicted proteins and molecular features of metabolic activities from several metagenomic samples. We suggest that the efficiency and sensitivity of this method will accelerate the decryption of microbial diversity and the signature of proteins and their metabolism from environmental samples.
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