Kisspeptins are new actors in the neuroendocrine regulation of reproduction. In vertebrates, the number of kiss genes varies from none to three. Zebrafish have two kiss genes, kiss1 and kiss2, and two kiss receptors (GPR54), kiss1r and kiss2r. To provide detailed information on the organization of the kiss systems in zebrafish, antibodies were raised against the C terminus of zebrafish preproKiss1 and preproKiss2. Immunohistochemistry fully confirmed in situ hybridization data, showing that kiss1-expressing neurons are only located in the habenular nucleus, while kiss2-expressing neurons are found in the dorsal and ventral hypothalamus. Kiss1-expressing cells project only to the interpeduncular and raphe nuclei and strongly expressed the kiss1r receptor. In contrast, kiss2-expressing cells are mostly present in the dorsal and ventral hypothalamus and project widely into the subpallium, the preoptic area, the thalamus, the ventral and caudal hypothalamus, and the mesencephalon. All these regions strongly expressed the kiss2r messengers. Kiss2 fibers profusely innervate the ventral forebrain and notably made close apposition with GnRH3 neurons. Estrogen treatment of juvenile fish with estradiol causes increase in kiss2 and kiss2r expression. In the pituitary gland, no proKiss2- positive fibers were detected, while positive cells were observed in the pars intermedia. In addition to proposing a successful strategy to develop antibodies to kisspeptins, these data indicate that the kiss2 systems of zebrafish are implicated in reproductive events, while the kiss1 gene would play other functions that remain to be established.

The establishment of international and regional standards for electric vehicle (EV) magnetic field wireless power transfer (MF-WPT) systems started in 2010 by the Society of Automotive Engineers (SAE). In the meantime, the EV MF-WPT standardization has been focused on primary device and secondary device topology. Recently, the International Organization for Standardization (ISO), the International Electrotechnical Commission (IEC), and SAE have begun describing the communication and alignment techniques for EV MF-WPT. In this paper, we present a fine positioning method using a low-frequency (LF) signal, as mentioned in IEC 61980 and SAE J2954. Through modeling and simulation, we optimized a LF ferrite rod antenna (FRA) for EV MF-WPT fine positioning. We also found the optimal arrangement of LF-FRAs on primary device and secondary device Finally, we used a test bench to experiment and check the results of our proposal.

In the last decade, engineers from automotive manufacturers and charging infrastructure suppliers have widely studied the application of wireless power transfer (WPT) technology to electric vehicles. Since this time, engineers from automotive manufacturers have studied precise positioning methods suitable for WPT using methods such as mechanical, communication-based or video-based. However, due to high costs, electromagnetic interference and environmental factors, the experts of the SAE J2954 was focused on the WPT’s precise positioning method by ferrite antennas and low power excitation. In this study, we present how to use the ferrite antennas to find a central alignment point between the primary and secondary units within the alignment tolerance area that requires the minimum power transfer efficiency of the EV WPT system. First, we analyze the ferrite antenna already applied in the automotive and verifies whether it is suitable for the precise positioning of the WPT system for EV. We use modeling and simulation to show that it is necessary to calculate all induced loop voltages in the relationship between incident magnetic field signal strength and induced loop voltage because of the short distance between the transmitter and receiver of the ferrite antenna in WPT. In addition, we also suggest a sequence to find the fitting location of the ferrite antenna, the number of antennas used and the center alignment point. After the simulation is performed on the suggestions, component-level and vehicle-level tests were conducted to verify the validity of the simulation results. As a result, it is shown that a ferrite antenna is suitable as a method for the secondary device to find the center alignment point of the primary device.