Cytochrome P450 (CYP) monooxygenases, the nature’s most versatile biological catalysts have unique ability to catalyse regio-, chemo-, and stereospecific oxidation of a wide range of substrates under mild reaction conditions, thereby addressing a significant challenge in chemocatalysis. Though CYP enzymes are ubiquitous in all biological kingdoms, the divergence of CYPs in fungal kingdom is manifold. The CYP enzymes play pivotal roles in various fungal metabolisms starting from housekeeping biochemical reactions, detoxification of chemicals, and adaptation to hostile surroundings. Considering the versatile catalytic potentials, fungal CYPs has gained wide range of attraction among researchers and various remarkable strategies have been accomplished to enhance their biocatalytic properties. Numerous fungal CYPs with multispecialty features have been identified and the number of characterized fungal CYPs is constantly increasing. Literature reveals ample reviews on mammalian, plant and bacterial CYPs, however, modest reports on fungal CYPs urges a comprehensive review highlighting their novel catalytic potentials and functional significances. In this review, we focus on the diversification and functional diversity of fungal CYPs and recapitulate their unique and versatile biocatalytic properties. As such, this review emphasizes the crucial issues of fungal CYP systems, and the factors influencing efficient biocatalysis.
New genus Upretia S. Y. Kondr., A. Thell and J. S. Hur is characterized by partly pruinose, lobate to subsquamulose, olivaceous grey to brown thallus, small ascospores and narrowly bacilliform conidia. It belongs to the subfamily Caloplacoideae of the Teloschistaceae and includes the ‘Caloplaca’ amarkantakana clade. The new genus is closely related to Ioplaca Poelt according to phylogeny analysis based on ITS1/ITS2 nrDNA, 28S nrLSU and 12S mtSSU sequences. The new combination Upretia amarkantakana is proposed Caloplaca amarkantakana Y. Joshi and Upreti.
Lichens are generally known as self-sufficient, symbiotic life-forms between fungi and algae/ cyanobacteria, and they also provide shelter for a wide range of beneficial bacteria. Currently, bacterial-derived biodegradable polyhydroxyalkanoate (PHA) is grabbing the attention of many researchers as a promising alternative to non-degradable plastics. This study was conducted to develop a new method of PHA production using unexplored lichen-associated bacteria, which can simultaneously degrade two ubiquitous industrial toxins, anthracene and naphthalene. Here, 49 lichen-associated bacteria were isolated and tested for PHA synthesis. During the GC-MS analysis, a potential strain of EL19 was found to be a 3-hydroxyhexanoate (3-HHx) accumulator and identified as Pseudomonas sp. based on the 16S rRNA sequencing. GC analysis revealed that EL19 was capable of accumulating 30.62% and 19.63% of 3-HHx from naphthalene and anthracene, respectively, resulting in significant degradation of 98% and 96% of naphthalene and anthracene, respectively, within seven days. Moreover, the highly expressed phaC gene verified the genetic basis of PHA m c l production under nitrogen starvation conditions. Thus, this study strongly supports the hypothesis that lichen-associated bacteria can detoxify naphthalene and anthracene, store energy for extreme conditions, and probably help the associated lichen to live in extreme conditions. So far, this is the first investigation of lichen-associated bacteria that might utilize harmful toxins as feasible supplements and convert anthracene and naphthalene into eco-friendly 3-HHx. Implementation of the developed method would reduce the production cost of PHA m c l while removing harmful waste products from the environment.
Adiponectin-synthesis-promoting compounds possess therapeutic
potential
to treat diverse metabolic diseases, including obesity and diabetes.
Phenotypic screening to find adiponectin-synthesis-promoting compounds
was performed using the adipogenesis model of human bone marrow mesenchymal
stem cells. The extract of the endolichenic fungus Daldinia
childiae 047215 significantly promoted adiponectin production.
Bioactivity-guided isolation led to 13 active polyketides (1–13), which include naphthol monomers, dimers,
and trimers. To the best of our knowledge, trimers of naphthol (1–4) have not been previously isolated
as either natural or synthetic products. The novel naphthol trimer
3,1′,3′,3″-ternaphthalene-5,5′,5″-trimethoxy-4,4′,4″-triol
(2) and a dimer, nodulisporin A (12), exhibited
concentration-dependent adiponectin-synthesis-promoting activity (EC50 30.8 and 15.2 μM, respectively). Compounds 2 and 12 bound to all three peroxisome proliferator-activated
receptor (PPAR) subtypes, PPARα, PPARγ, and PPARδ.
In addition, compound 2 transactivated retinoid X receptor
α, whereas 12 did not. Naphthol oligomers 2 and 12 represent novel pan-PPAR modulators
and are potential pharmacophores for designing new therapeutic agents
against hypoadiponectinemia-associated metabolic diseases.
Jae-Beom Lee et al. 535With video compression standards such as MPEG-4, a transmission error happens in a video-packet basis, rather than in a macroblock basis. In this context, we propose a semantic error prioritization method that determines the size of a video packet based on the importance of its contents. A video packet length is made to be short for an important area such as a facial area in order to reduce the possibility of error accumulation. To facilitate the semantic error prioritization, an efficient hardware algorithm for face tracking is proposed. The increase of hardware complexity is minimal because a motion estimation engine is efficiently re-used for face tracking. Experimental results demonstrate that the facial area is well protected with the proposed scheme.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.