molecules of other serotypes, significantly lower than the sequence identities of between 93 and 100 % observed among other serotypes characterized to date. This was consistent with previous observations that there were significant immunological differences between and other BTV serotypes and that monoclonal antibodies raised against BTV-1 VP7 failed to react with BTV-15 VP7. Recombinant BTV-15 VP7 protein produced from Eseheriehia coil was largely insoluble, but maintained its immunogenicity. Polyclonal mouse sera raised against the recombinant VP7 protein reacted strongly with VP7 of BTV-15, but weakly with that of BTV-1.
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