An engineered cyanovirin‐N homologue that exhibits specificity for high mannose N‐glycans has been constructed to aid type I α 1,2‐mannosidase inhibitor discovery and development. Engineering the lectins C‐terminus permitted facile functionalization with fluorophores via a sortase and click strategy. The resulting lectin constructs exhibit specificity for cells presenting high mannose N‐glycans. Importantly, these lectin constructs can also be applied to specifically assess changes in cell surface glycosylation induced by type I mannosidase inhibitors. Testing the utility of these lectin constructs led to the discovery of type I mannosidase inhibitors with nanomolar potency. Cumulatively, these findings reveal the specificity and utility of the functionalized cyanovirin‐N homologue constructs, and highlight their potential in analytical contexts that require high mannose‐specific lectins.
Entre el converso Sebastián de Horozco y sus dos hijos Juan de Horozco y Covarrubias y Sebastián de Covarrubias y Horozco, había antagonismos familiares. En parte estos conflictos surgían porque como conversos no podían ocupar puestos que exigían limpieza de sangre. La amargura de los Horozco se intensifica aun cuando otros deudos — en particular los Hegas — por ser tenidos como cristianos viejos, podían disfrutar de todas las ventajas de su estado social.
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