An engineered cyanovirin‐N homologue that exhibits specificity for high mannose N‐glycans has been constructed to aid type I α 1,2‐mannosidase inhibitor discovery and development. Engineering the lectins C‐terminus permitted facile functionalization with fluorophores via a sortase and click strategy. The resulting lectin constructs exhibit specificity for cells presenting high mannose N‐glycans. Importantly, these lectin constructs can also be applied to specifically assess changes in cell surface glycosylation induced by type I mannosidase inhibitors. Testing the utility of these lectin constructs led to the discovery of type I mannosidase inhibitors with nanomolar potency. Cumulatively, these findings reveal the specificity and utility of the functionalized cyanovirin‐N homologue constructs, and highlight their potential in analytical contexts that require high mannose‐specific lectins.
Entre el converso Sebastián de Horozco y sus dos hijos Juan de Horozco y Covarrubias y Sebastián de Covarrubias y Horozco, había antagonismos familiares. En parte estos conflictos surgían porque como conversos no podían ocupar puestos que exigían limpieza de sangre. La amargura de los Horozco se intensifica aun cuando otros deudos — en particular los Hegas — por ser tenidos como cristianos viejos, podían disfrutar de todas las ventajas de su estado social.
Scholars have long noted the influence of Russian writers, particularly Tolstoy and Dostoevsky, on the novels of Benito Pérez Galdós. However, in an interview granted to the Russian journalist la. Pavlovskii in 1884 (only recently published in the West) Galdós acknowledges an indebtedness to an earlier Russian master, Ivan Turgenev, referring to him as “my great teacher.” A close reading of Turgenev's Fathers and Sons and Galdós' Doña Perfecta suggests that the Russian masterpiece may have inspired the well-known Spanish work. The novels share a common theme (the conflict between generations), and in each the hero is a young man trained in science who makes an extended visit to the provinces. In both works the protagonist dies a tragic, untimely death. There are other important similarities between the two books, but significant differences as well, particularly in the authors' attitudes toward the conflict itself. Galdós believed completely in Pepe Rey's cause, while Turgenev was ambivalent about the forces his hero represented; thus, in tone and structure each novel reflects its author's feelings. There appears to be no doubt that Galdós knew Fathers and Sons and made use of certain of its ideas in creating his own independent and highly personal interpretation of the conflict between generations.
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