Hamstring strain injuries (HSIs) remain the most prevalent cause of time lost from competition in a range of sports, 14,17,18,53 with associated performance 23 and financial consequences. 28 Deficits in function, such as reduced isometric knee flexor strength, exist acutely following HSI 4,44,58 and may increase reinjury risk if persistent at return-to-play (RTP) clearance. 16 Rehabilitation should aim to restore these deficits as quickly as possible following acute HSI and to return the injured athlete to his or her sport with minimal risk of reinjury. 26 However, even after completion of rehabilitation and RTP clearance, previously injured hamstrings may display eccentric strength 42,49,51,74 and biceps femoris long head (BFLH) fascicle length deficits, 73 which are both modifiable HSI risk factors. 11,52,71,79 Fyfe et al 22 hypothesized that a lack of eccentric loading and longlength exercise during early rehabilitation may contribute to residual deficits and the elevated risk of reinjury seen in previously injured hamstrings. 20,22,50 Eccentric loading and long-length exercises reduce HSI risk, 3,77,80 increase knee flexor strength and BFLH fascicle length in uninjured individuals, 1,10,54,55,72 and accelerate RTP time when emphasized during rehabilitation. 5,6 However, the introduction and progression of eccentric loading and long-length exercises may be delayed by the consistently implemented guideline to only perform and progress exercise in the absence of pain. 30 Delaying the start of exercise rehabilitation by 9U OBJECTIVE: The primary aim was to compare time from acute hamstring strain injury (HSI) to return-to-play (RTP) clearance following a standardized rehabilitation protocol performed within either pain-free or pain-threshold limits. Secondary aims were to compare isometric knee flexor strength, biceps femoris long head (BFLH) fascicle length, fear of movement, and reinjury occurrence at the 6-month follow-up between pain-free and pain-threshold groups.U DESIGN: Randomized controlled trial.U METHODS: Forty-three men with acute HSIs were randomly allocated to a pain-free (n = 22) or painthreshold (n = 21) rehabilitation group. Days from HSI to RTP clearance, isometric knee flexor strength, BFLH fascicle length, fear of movement, and reinjury occurrence at the 6-month follow-up were reported.U RESULTS: Median time from HSI to RTP clearance was 15 days (95% confidence interval [CI]: 13, 17) in the pain-free group and 17 days (95% CI: 11, 24) in the pain-threshold group, which was not significantly different (P = .37). Isometric knee flexor strength recovery at 90° of hip and 90° of knee flexion was greater in the pain-threshold group at RTP clearance by 15% (95% CI: 1%, 28%) and by 15% (95% CI: 1%, 29%) at 2-month follow-up, respectively. Improvement in BFLH fascicle length from baseline was 0.91 cm (95% CI: 0.34, 1.48) greater at 2-month follow-up in the pain-threshold group. Two reinjuries occurred in both the pain-free and pain-threshold groups between RTP clearance and the 6-month follow-up.U CON...