The microstructure of leaves and ultrastructure of chloroplasts were examined in tomato (Lycopersicon esculentum L.) plants treated with elevated temperature. Plants were exposed to 35ºC for 30 d after florescence. The plants grown continuously under 25ºC served as controls. Compared with the controls, the net photosynthetic rate (P N ) in stressed plants decreased significantly. Stomatal conductance, intercellular CO 2 concentrations, the rate of transpiration, and the limitation of stomatal conductance showed that the decrease in P N was caused mainly by nonstomatal restrictions. Meanwhile, stomata density increased significantly in the stressed plants. The stomata status of opening and closing became disorganized with a prolonged 35ºC exposure. The damage of chloroplast membrane occurred earlier and was more serious in the plants under elevated temperature. At the same time, the thylakoids were loosely distributed with lesser grana, but the number of lipid droplets increased in chloroplasts. The number of starch grains in chloroplasts increased first and then decreased. In addition, the length of the main nerve in leaves increased and the main vein showed distortion in the plants stressed by 35ºC. An increase was observed in the number of cells on the abaxial side of the main vein and these cells were overly congregated. The thickness of a vertical section became thinner in the stressed leaves. The cells of the upper epidermis thinned, and the ratio of palisade tissue to spongy tissue decreased. Generally, the photosynthetic apparatus of tomato changed significantly and the changed chloroplast ultrastructure might be one of the important reasons that caused the decrease of PN under 35ºC.
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