The objectives of this work were to develop an antibiotic coating on the surface of a titanium plate to determine its antibacterial properties in vitro and in vivo. To prepare vancomycin-coated titanium implants, we adopted the electrospinning nanotechnique. The surface structure of the coating implants was observed using a scanning electron microscope. An elution method and a high-pressure liquid chromatography assay were used to characterize the release behavior of vancomycin from the coating. The antibacterial efficacy and the cytotoxicity of the coated titanium implants on osteoblasts were investigated in vitro. In addition, X-ray, white blood cell count, C-reactive protein, erythrocyte sedimentation rate, and pathological examination were performed to validate its antimicrobial efficacy in vivo. The antibiotic coating released 82.7% (approximately 528.2 μg) of total vancomycin loading in the coating in vitro. The release behavior of vancomycin from nanofiber coatings exhibited a biphasic release pattern with an initial burst on day 1, followed by a slow and controlled release over 28 days. There was no cytotoxicity observed in vitro for the vancomycin-loaded coating. The vancomycin-coated titanium implants were active in treating implant-associated infection in vivo. Thus, vancomycin-coated titanium implants may be a promising approach to prevent and treat implant-associated infections.
Polyethylene terephthalate in textile form (Dacron) has been used extensively as a surgical implant material for applications such as vascular grafts and percutaneous access devices. It is moderately histocompatible eliciting a chronic inflammatory reaction predominately in tissue which has grown into the pores of the fabric. Titanium implants on the other hand, induce only the slightest inflammatory response and connective tissue adhesion to the titanium oxide surface is excellent. It was, therefore, hypothesized that a titanium coating on Dacron fabric might improve its histocompatibility while leaving its desirable mechanical properties unaffected. To test this idea, Dacron velour specimens were coated with titanium by vacuum deposition and were implanted together with uncoated controls in rabbits subcutis. After various implantation times, the specimens were recovered and the associated tissue was examined histologically. Qualitative and semiquantitative analysis revealed that tissue ingrowth quantity and quality was highly variable, not only between test and control specimens, but also between animals and even between specimens within the same animal. This indicated that there may be a number of factors influencing tissue ingrowth that were not adequately controlled in this study. The titanium coating which was undoubtedly highly oxidized had a profound qualitative and quantitative effect on fibroblast activity (ground substance formation) and fibroblast adhesion to the Dacron fibers. The results obtained after these short-term implantation periods indicate that titanium coating affects the quality of the interfacing tissue and may actually improve long-term histocompatibility. Long-term studies will have to confirm these preliminary data.
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