The vanilloid receptor (VR1) protein functions both as a receptor for capsaicin and a transducer of noxious thermal stimuli. To determine the expression and targetting of this protein, we have generated antisera against both the amino and carboxy termini of VR1. Within the dorsal root and trigeminal ganglia of rats, VR1-immunoreactivity (VR1-ir) was restricted to small and medium sized neurons. VR1-ir was transported into both the central and peripheral processes of these primary afferent neurons, as evidenced by: (i) the presence of VR1-ir in nerve fibres and terminals in lamina I and lamina II of the superficial dorsal horn, and the association of VR1-ir with small diameter nerve fibres in the skin and cornea; (ii) the reduction of VR1-ir in the spinal cord after dorsal rhizotomy; and (iii) the accumulation of VR1-ir proximal to sciatic nerve ligation. At the ultrastructural level, VR1-ir was associated with plasma membranes of neuronal perikarya in dorsal root ganglia and nerve terminals in the dorsal horn. VR1-ir was also seen in nerve fibres and terminals in the spinal trigeminal nucleus and nucleus of the solitary tract. Within a large proportion of dorsal root ganglion neurons and the terminals of their axons, VR1-ir was colocalized with staining for the P2X3 purinoceptor, and with binding sites for the lectin IB4. Surprisingly, VR1-ir did not coexist substantially in nerve fibres and terminals that contain substance P and calcitonin gene-related peptide, suggesting complex mechanisms for the release of these neuropeptides in response to capsaicin application.
The cloned vanilloid receptor VR1 can be activated by capsaicin and by thermal stimuli. The pattern of nerve terminals that contain VR1 in adult rat spinal cord does not correspond to axons that arise from a single subset of dorsal root ganglion neurons. Thus, we postulated that the basis underlying this complexity might be better understood from a developmental perspective. First, using capsaicin-induced hyperalgesia as a measure of VR1 function, we found that vanilloid receptors were functional as early as postnatal day 10 (P10), although hyperalgesia was of longer duration in adult. Interestingly, the appearance of VR1 protein in terminals of dorsal root ganglion neurons shifts over this postnatal period. From embryonic day 16 to P20, the majority of VR1 protein in the spinal cord was observed in lamina I. As animals matured, VR1 protein became more abundant in lamina II, particularly in the inner portion. Consistent with these observations, the number of dorsal root ganglion neurons coexpressing VR1 and isolectin B4 binding sites doubled while the number of neurons that had both VR1 and substance P remained relatively constant from P2 to P10. In peripheral processes, the number of VR1-positive nerve fibres and terminals in cutaneous structures in postnatal day 10 was half of that in adults. We also show that the association of VR1 with Ret is the reciprocal of the association of VR1 with Trk A. These results suggest that neurotrophins may regulate the extent to which populations of dorsal root ganglion neurons express VR1.
This paper describes a numerical procedure for solving two-dimensional elastostatics problems with multiple circular holes and elastic inclusions in a finite domain with a circular boundary. The inclusions may have arbitrary elastic properties, different from those of the matrix, and the holes may be traction free or loaded with uniform normal pressure. The loading can be applied on all or part of the finite external boundary. Complex potentials are expressed in the form of integrals of the tractions and displacements on the boundaries. The unknown boundary tractions and displacements are approximated by truncated complex Fourier series. A linear algebraic system is obtained by using Taylor series expansion without boundary discretization. The matrix of the linear system has diagonal submatrices on its diagonal, which allows the system to be effectively solved by using a block GaussSeidel iterative algorithm.
Our objectives were to determine the acceptability of irradiated fresh ground beef and to determine whether the acceptability was affected by information about benefits or by identifying the samples. The 218 subjects were separated into 4 groups comprising a 2 ϫ ϫ ϫ ϫ ϫ 2 design of benefits and information. All subjects tasted 4 patties: 2 irradiated and 2 control. Ratings of overall liking, toughness, and flavor and texture liking were equal for both. Subjects rated the irradiated beef patties juicier than the nonirradiated. Benefit information and sample identification increased the liking ratings of the patties-because the group with no benefit information and no sample identification generally rated all samples lower.
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