The changes of corrosion potential (E corr ) of metals immersed in seawater were investigated with electrochemical technology and epifluoresence microscopy. In natural seawater, changes of E corr were determined by the surface corrosion state of the metal. E corr of passive metals exposed to natural seawater shifted to noble direction for about 150 mV in one day and it didn't change in sterile seawater. The in-situ observation showed that biofilms settled on the surfaces of passive metals when E corr moved in noble direction. The bacteria number increased on the metal surface according to exponential law and it was in the same way with the ennoblement of E corr . The attachment of bacteria during the initial period played an important role in the ennoblement of E corr and it is believed that the carbohydrate and protein in the biofilm are reasons for this phenomenon. The double layer capacitance (C dl ) of passive metals decreased with time when immersed in natural seawater, while remained almost unchanged in sterile seawater. The increased thickness and reduced dielectric constant of C dl may be reasons.
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