Summary.A method for the isotope dilution-mass spectrometric (ID-MS) determination of butyric acid C4 in butter fat (RM164) was developed in order to support the data gathered from nine experienced European laboratories within the final certification exercise. The ID-MS results (3,46 _+ 0,06 g C4/100 g fat) were in very good agreement with those obtained by "classical" GLC and HPLC techniques. (RM164 was finally certified at 3,49 + 0,06 g C4/100 g fat). This paper reports briefly on a previous preliminary study undertaken to validate a procedure (agreed by the BCRsterol group) for the isolation of the sterol from fats and oils. By use of labelled sterols and radiometric measurements it was shown that sterol recoveries were superior to 96%.The procedure was applied during the 3rd Intercomparison exercise for sterol determination in RM162 (Blend of Soya-Maize oil), for the GLC measurements of cholesterol in RM380 (Whole milk powder) and RM384 (Lyophilized pork muscle) and to the ID-MS determination of cholesterol in RM163 (blend of animal fats) and RM164 (anhydrous milk fat).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.