The effect of ultraviolet-B (280 to 320 nm) irradiation on physiological activities of Anabaena flos-aquae and the water fern Azolla caroliniana has been studied under conditions where lethal effects of irradiation are absent. Nitrogenase activity, measured as acetylene reduction, specifically declined during irradiation with low levels of ultraviolet-B, whereas other physiological activities were unaffected. These findings indicate that measurement of acetylene reduction by cyanobacteria may serve as a specific, sensitive biochemical assay to assess environmental ultraviolet-B effects due to depletion of stratospheric ozone. Photobiol. 15:269-274. 12. Witken, E. M. 1976. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol. Rev. 40:869-907.
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