At the initiation of lactation, Ca homeostatic mechanisms have to react to a tremendous increase in demand for Ca. Mobilization of Ca from bone and increased absorption from the gastrointestinal tract are required to re-establish homeostasis. It has been shown that dietary anions play an important role in the prevention of milk fever by mobilizing Ca from bone and by increasing Ca absorption in the GI tract. The purpose of the present study was to investigate the influence of different Ca contents in diets supplemented with anionic salts on bone metabolism of dairy cows. Twenty-four holstein cows (housed inside, second to fourth lactation) without a milk fever history were divided into four groups (A, B, C, D). Each group was fed a different diet which was given from day 263 of gestation till the day of parturition. Group A and B received a low calcium diet (4 g/kg DM) whereas group C and D received a high Ca diet (8 g/kg DM). In addition group B and D received anionic salts. The DCAD was calculated with the formula: DCAD (mEq/kg DM)=(0.2 Ca2++0.16 Mg2++Na++K+)-(Cl-+0.6 S2-+0.65 P3-). Blood and urine samples were collected on days 256, 270 and 277 of gestation, on the day of parturition as well as the following 5 days and on days 9, 14 and 19 after parturition. Serum Ca, P, Mg, ICTP, OC, VITD, PTH and urinary pH were analysed. The bone resorption marker ICTP showed a significant increase after parturition in all the groups. On the contrary, the bone formation marker OC decreased after parturition in all the groups. The VITD concentrations in group D and the urinary pH in group B were significantly lower compared to the other groups (p<0.05). The Ca concentrations tended to be higher in group B around parturition than in all the other groups. No significant influence of the four different diets on all the other parameters could be shown. In conclusion, this data showed that the addition of anions and the different Ca contents had no significant influence on bone resorption and bone formation markers. This may be because of the fact that the dietary cation-anion balance was not low enough (DCAD-group A: 181 mEq/kg DM, group B: -48 mEq/kg DM, group C: 210 mEq/kg DM and group D: 28 mEq/kg DM) to induce a metabolic acidosis with all its positive effects on calcium metabolism.

The objective of this research was to determine the optimal level of an encapsulated butyric acid (ButiPEARL) based on the performance of male Cobb broilers reared to 42 d of age and to investigate its effects on intestinal morphology. Experiment 1 ( EXP 1: ) consisted of 4 treatments with 12 replicate pens that contained 45 broilers, and Experiment 2 ( EXP 2: ) consisted of 6 treatments with 8 replicate pens that contained 50 broilers. Birds were weighed by pen on d 0, 21, 35, and 42. In EXP 1, the treatments were as follows: 1) control ( C: ); 2) C + 100 g ButiPEARL/ton; 3) C + 200 g ButiPEARL/ton; and 4) C + 300 g ButiPEARL/ton. In EXP 2, the treatments were identical to EXP 1, with 2 additional treatments: 5) C + 400 g ButiPEARL/ton and 6) C + 500 g ButiPEARL/ton. In EXP 1, two 42-d-old broilers per pen were randomly selected for duodenal and jejunal tissue collection. Only the samples from the broilers fed the C or 300 g ButiPEARL treatments were analyzed for histology in EXP 1. For EXP 2, on d 21 and 35, two broilers per pen were randomly selected for duodenal, jejunal, and ileal tissue collection. For EXP 1 and 2, BW gain increased linearly with increasing butyric acid levels (P < 0.027 and P < 0.001, respectively). For EXP 1 and 2, feed conversion linearly improved with increasing butyric acid from 0 to 42 d (P < 0.001 and P < 0.001, respectively). In EXP 1, there were no differences in any intestinal morphology at 42 d between broilers fed the C or 300 g ButiPEARL treatments. In EXP 2, there were no differences in villus height at 21 or 35 d of age with any level of butyric acid. Based on the results of this research related to BW gain and feed conversion, the recommended optimum dosage level for ButiPEARL in broilers reared to 42 d of age is up to 500 g/ton.

Data for individual feed intake, liveweight, and egg production were recorded for five genetically different groups of 40 pullets each during 10 test periods of 28-days each. Average ambient temperature at cage level varied from 6.7 C during January to 21.1 C during June. A 2890 kcal/kg metabolizable energy (ME) diet with 16% crude protein (CP) was fed ad lib. Average egg production for the Small Leghorns (SL) was about 60%, for white-egg hybrids (WL) about 75%, for brown-egg hybrids (BL) about 72%, for female line broiler breeders (BB) about 48%, and for broiler-cross pullets (B) about 51%. Average grams liveweight and grams feed intake per hen day were: SL, 1426 and 83.7; WL, 1809 and 104.9; BL, 2610 and 122.9: BB, 4197 and 156.2; and B, 4158 and 167.7.Partition equations which describe the data for SL, WL, BL, and BB, assuming 70% efficiency of use of feed ME for maintenance, tissue formation, and egg formation were: F = (.534 -.004T)W .653 + 2.76AW + .80EM and F = (.259 -.00259T)W-75 + 2.76AW + .80EM. Similar equations for B, assuming 65% efficiency use of ME, are: F = (.589 -.0044T)W°5 3 + 2.9AW + .85EM and F = (.275 -.00275T)W-75 + 2.9AW + .85EM. The terms are: F = grams feed/hen day; T = ambient temperature in °C; W = grams liveweight; AW = grams daily change in liveweight; EM = grams egg mass per hen day.Equations which assume 70% efficiency of use of ME are shown to predict feed intake of a diet containing 2890 kcal of apparent ME per kilogram for white and brown-egg layers and broilerbreeder pullets varying in individual body weights from about 1 to 5 kg. Equations, assuming 65% energetic efficiency, describe feed intake for a group of broiler-cross pullets. (

Intact and cecectomized adult male, Single Comb White Leghorns were fasted for 24 hr prior to the initiation of the collection period. Excreta were quantitatively collected from each of the unfed control roosters for a 24 to 48 hr fasting period. The amino acids excretion values (mg/bird/24 hr) were not significantly different (P greater than .05) between the intact and cecectomized roosters for histidine or methionine. However, lysine and glutamic acid were significantly different (P less than .05) and all other reported amino acids were significantly different (P less than .01). The results of this preliminary experiment indicate the possible role of the ceca in altering the amino acid excretion values. Additional research is required in the elucidation of cecal involvement, as well as the selection of a proper control for this method of analysis for amino acid availability.