The tissue disposition of the off-flavor compound 2-methylisoborneol (MIB) was examined in market-sized channel catfish (Ictalurus punctatus). Purified MIB was intravascular administered to channel catfish at a dosage of 1 mg∙kg−1 body weight. Body tissues and fluids were collected at intervals following administration and were analyzed for MIB by microwave distillation and capillary gas chromatography. Plasma clearance of MIB was characterized by a two-compartment open model with half-lives of 0.14 and 3.62 h or distribution and elimination phases, respectively. MIB was more concentrated in peritoneal fat and subepidermal adipose tissue than in other tissues. The concentration in the edible flesh decreased from 0.107 μg∙g−1 at 2 h to 0.025 μg∙g−1 at 96 h. There was no evidence of biotransformation of MIB to the related compounds 2-rnethylenebornane and 2-methyl-2-bornene over the 96-h sampling period. The low recoveries of the administered dose in body fluids and tissues and the rapid clearance suggested significant gill excretion of this compound.
Fenbendazole (FBZ) was administered intravenously (1 mg/kg) and orally (5 mg/kg) to catheterized, confined channel catfish. Blood samples were collected for 72 h, and resulting FBZ plasma concentrations were pharmacokinetically modelled. Following intravenous administration t 1/2 alpha was 0.51 h, t 1/2 beta was 16.8 h, body clearance (C1B) was 0.0598 L/kg/h, and Vd (area) was 1.45 L/kg. After oral administration the t 1/2 (abs) was 1.47 h, the t 1/2 beta was 20.1 h, and the tlag was 0.1 h. Following oral administration of 5 mg FBZ/kg body weight, the following tissues and body fluids were sampled for concentrations of FBZ, oxfendazole (FBZ-SO), sulphone metabolite (FBZ-SO2) and hydroxy metabolite (FBZ-OH): liver, posterior kidney, fat, muscle, bowel contents and urine. Fenbendazole was detected in the highest concentrations in abdominal fat, whereas oxfendazole was found primarily in the kidney, liver and abdominal fat. The sulphone metabolite was detected only in urine and bowel contents, while the hydroxy metabolite was found most often in the liver and abdominal fat samples.
Volumes of plasma, extracellular fluid, and total body water were estimated with indicator-dilution techniques in the adult channel catfish (Ictalurus punctalus). The experiments were performed with catheteri/.cd, nonsedated channel catfish restrained in flowthrough, Plexiglas environmental chambers. Marker substances used to estimate body fluid volumes were Evans blue for plasma, sodium thiocyanate for extracellular fluid, and antipyrine for total body water. For comparisons, total body water was also estimated by desiccating fish to a constant weight. Plasma volume was determined to be 28.1 ± 6.2 mL/kg body weight (mean ± SD), extracellular fluid volume was 183.2 ± 34.6 mL/ kg, and total body water was 531.3 ± 76.4 mL/kg. Total body water volume estimated by desiccation was 664.1 ± 50.8 mL/kg.
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