The physicochemical properties of honey vary considerably depending on the nectar sipped by Apis mellifera bees because these characteristics are determined by environmental, geographical, and vegetational conditions in foraging areas. The aim of this study was to characterize types of honey from the Biobío Province in the Biobío Region, Chile, according to its botanical origin and physicochemical and microbiological characteristics, and thus contribute to increased knowledge about honey in the region. A total of 11 samples from different communes within the region were analyzed. Physicochemical parameters such as moisture, insoluble solids, ashes, electrical conductivity, pH, and hydroxymethylfurfural were determined, and the presence of coliforms and Escherichia coli was evaluated. Two places of provenance were characterized as monofloral of introduced plant species: the M11 sample of Melilotus indicus and the M1 sample of Echium vulgare. One place of provenance (M6 sample) was characterized as monofloral of Eucryphia glutinosa, species endemic to Chile. All types of honey complied with the physicochemical standards established by national and international food safety regulations, and none had any signs of coliforms or E. coli. Results showed that there are new types of honey in the region that meet all the required parameters; in addition, they provide a comparison of plant species that are unique in the world. This demonstrates the potential of these types of honey for national and international marketing.
Abstract:Holm oak (Quercus ilex L.), a typical evergreen tree of the Mediterranean area, is very important due to its ecological and economical values. Propagation of this species is extremely difficult and traditionally carried out only by seed germination. In this work, mature acorns were germinated in vitro and in peat substrate in aseptic and non-aseptic conditions. Explants from the seedlings obtained were propagated in vitro in WPM plus 4 µM BA. Plant regeneration was achieved from hypocotyls and root segments cultured in vitro on modified Gamborg medium plus 20 µM BA and 20 µM NAA. 13.8% of the hypocotyls and approximately 30% of the root segments developed both shoots and roots after 30 days of culture. Rooting of stem segments was obtained both in vitro and ex vitro by basal dipping in IBA solutions. Within ex vitro rooting, mother plant age had major influence on the percentage of rooting of the cuttings as the younger plants showed higher ability to root. In this way, Q. ilex plants could be propagated and cloned. The procedure described here would be a very useful tool for breeding programs since vegetative propagation of selected individuals can be achieved.
In vitro culture methods were used to germinate olive embryos prior to maturation. Fruit, seed and embryo development were established with consecutive sampling from 20 to 100 days after bloom. For that same period, embryo development and germination success were determined by in vitro culture trials using one-third strength MS medium with or without the addition of zeatin. For early developmental stages, when isolation of the embryo was difficult, a cut portion of the seed containing the embryo was used for culture. The embryos cultured within the cut seed portions germinated and formed normal plantlets. Histological observations indicated a close similarity between the natural and in vitro immature embryo differentiation pattern, progressing through preglobular, globular, heartshaped and torpedo-shaped stages. In some cases, however, the in vitro immature embryos developed or germinated abnormally. The presence of zeatin (0.25 mg 1-1) in the culture medium and the use of a cut seed-portion containing the immature embryo allowed in vitro germination sooner after bloom than previously obtained. On the contrary, zeatin was a handicap for mature olive embryo in vitro germination, which reached 100% seedling formation when no plant growth regulators were used.
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