Oxygen (O2) is life essential but as a drug has a maximum positive biological benefit and accompanying toxicity effects. Oxygen is therapeutic for treatment of hypoxemia and hypoxia associated with many pathological processes. Pathophysiological processes are associated with increased levels of hyperoxia-induced reactive O2 species (ROS) which may readily react with surrounding biological tissues, damaging lipids, proteins, and nucleic acids. Protective antioxidant defenses can become overwhelmed with ROS leading to oxidative stress. Activated alveolar capillary endothelium is characterized by increased adhesiveness causing accumulation of cell populations such as neutrophils, which are a source of ROS. Increased levels of ROS cause hyperpermeability, coagulopathy, and collagen deposition as well as other irreversible changes occurring within the alveolar space. In hyperoxia, multiple signaling pathways determine the pulmonary cellular response: apoptosis, necrosis, or repair. Understanding the effects of O2 administration is important to prevent inadvertent alveolar damage caused by hyperoxia in patients requiring supplemental oxygenation.
The purpose of this article is to describe proteomics, to discuss the importance of proteomics, to review different methods for protein measurement, and to illustrate how knowledge of proteomics might improve patient care. Among common laboratory determinations are those involving enzymatic (protein) function. Although the presence or activity of proteins may be seen clinically as incidental, proteins represent the engines through which critical life processes ensue. A selected review of the literature is presented to define and explain proteomics and to review the various techniques to measure proteins. A case-study approach is used to illustrate how proteomics can be utilized for detecting and monitoring disease. The human genome has been completely sequenced and proteomics has emerged as a way to unravel the biochemical and physiological mechanisms of diseases at the functional level. This review includes the recent discoveries regarding proteomics and its importance in the detection and treatment of disease.
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