Summary The effect of starvation for four days on the metabolism of antipyrine was studied in calves by measuring the antipyrine plasma clearance as well as the excretion of three major metabolites of antipyrine in urine. The study was carried out on 20 calves, females of the Holstein‐Friesian breed, (10 calves in a control group and 10 in an experimental group), aged 14–15 days. Antipyrine studies were performed in a experimental group before and after the 4 days of starvation. The starvation for four days was associated with a statistically significant (p < 0.01) increase in the plasma antipyrine elimination half‐life. In the starved calves the systemic clearance of antipyrine was significantly (p < 0.05) decreased. The excretion of the three major metabolites of antipyrine: 4‐hydroxyantipyrine, 3‐hydroxymethylantipyrine and norantipyrine in urine was significantly (p < 0.01) decreased after starvation. In the control group no significant differences between the pharmacokinetic parameters of antipyrine in 14–15 and 18‐19 day‐old calves were observed and the urinary profiles of antipyrine in calves from the control group did not differ significantly between 14–15 and 18–19 days of age. The data obtained from this study indicate that starvation for four days inhibits antipyrine elimination in calves. Zusammenfassung Der Einfluß einer viertägigen Hungerperiode auf den Antipyrinmetabolismus bei Kälbern In der vorliegenden Untersuchung sollte der Einfluß einer viertägigen Hungerperiode auf die Antipy‐rinclearance im Plasma, die Ausscheidung über die Nieren und die Nierenclearance von drei Antipyrin‐metaboliten bei Kälbern untersucht werden. Als Versuchstiere dienten 20 weibliche Holstein‐Friesian Kälber im Alter von 14–15 Tagen. Die Kälber wurden in zwei Grüppen eingeteilt (10 Kälber in der Kontrollgruppe und 10 Kälber in der Versuchsgruppe). Der Antipyrintest wurde 0 Tage und 4 Tage nach Nahrungsentzug durchgeführt. Nach viertägiger Hungerperiode war die Antipyrinhalbwertszeit (p < 0.01) wesentlich verlängert. Die Plasma‐Antipyrin‐Clearance, die Nierenausscheidung und die Nierenclearance der Antipyrin‐metabolite wurde (p < 0.01) bei viertägigem Nahrungsentzug bei den Kälbern deutlich reduziert. Aus der Kontrollgruppe geht hervor, daß sich hinsichtlich der Antipyrinhalbwertszeit, der Antipyrin‐clearance sowie der Nierenausscheidung keine wesentlichen Unterschiede zwischen 14–15 Tage alten und 18–19 Tage alten Kälbern (p > 0.05) zeigen. Ein viertägiger Nahrungsentzug reduziert demnach den Antipyrin‐Stoffwechsel wesentlich.
The purpose of this study was to test a modification of antipyrine test in calves. The experiment was carried out on 10 calves (bulls of black and white breed). Each subject received on days 10 and 20 of life. respectively. 15 mg.kg· l body mass of antipyrine as an intravenous bolus injection. followed by its oral administration "in substantia" in gelatin capsules 48 hours later. The concentrations of antipyrine in their saliva were repeatedly determined. Pharmacokinetics of antipyrine was followed in each subject (according to a one-compartment open model) using five modifications: a complete study (A). and four abbreviated studies. i.e using the 4. 8 and 12 h saliva collection times (B). the 4 and 8 h collection times (C), further the 4 and 12 h (D). and finally the 8 and 12 h saliva collection times (E). Correlational analysis was used to compare the substance kinetics from the complete set of data obtained from all saliva collections with the results using each of the abbreviated approaches.Based on the complete kinetics studies, mean variables for antipyrine were as follows: half-life In conclusion. we demonstrated that antipyrine pharmacokinetic parameters in calves can be determined with reasonable precision using a two-point or three-point saliva sampling procedure following a single oral dose. Moreover. this simplified sampling method can be used for largescale investigations. where estimation of hepatic mixed function oxygenases (MFO) activity is needed.Antipyrine, intravenous and oral administration. MFO system, calves
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