Callus initiated from bulb-scale expiants of the interspecific hybrid Lilium ‘Black Beauty’ on a Linsmaier-Skoog (LS) semisolid medium containing 4 mg/liter 2,4-dichloro-phenoxyacetic acid (2,4-D) was transferred to liquid LS with or without 4 mg/liter 2,4-D and incubated in darkness on a gyrorotary shaker at 100 rpm. Every 7 to 10 days the callus was transferred to fresh medium. The culture was maintained for 3 years. Plants were generated from the callus on semisolid LS at 1 year and 30 months after initiation of the liquid culture. Differentiation began with root formation, then shoots were produced. Supplementing the semisolid medium with 0.3 mg/liter α-naphthaleneacetic acid (NAA) stimulated root formation but shoot production was best on medium without growth regulators or medium containing 0.03 mg/liter NAA. Eleven of the 58 plants recovered (19%) bore variegated foliage but none of the 40 which flowered, including 2 with variegated leaves, deviated from the floral phenotype of ‘Black Beauty’.
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