This study is focused on the evaluation of dependable and eco-friendly methods for the synthesis of metal nanoparticles is a significant step in the area of application of nanotechnology. One of the alternatives to obtain this purpose is to use natural techniques such as biological approach. Here, we examine biosynthesis of metallic nanoparticles using extract Proteus sp. the metal nanoparticles were successfully synthesized via reduction of silver sulfate employed extracted cell of bacterium Proteus sp. Nevertheless, the extracellular acts as a reducing agent to convert silver ion from its aqueous solution and the synthetic were formed within 2 hrs. On the other hand, scanning electron microscopy (SEM) which describes the surface morphology of bio-reduction of Ag-nanoparticles demonstrated that the spherical nature occurred through the bio-synthesis process and the particles are mostly circular and irregular in shape, UV-visible exhibit a peak at 423 nm corresponding to the plasmon of silver nanoparticle and XRD pattern was taken and presented that all peaks were indexed by hexagonal wurtzite phase (PIXcel 1D). In spite of that, the band gap energy measured (2.93 eV) and suggested strong scattering of the X-ray in the crystalline phase. Finally, we concluded that this study offers the remarkable report that biological synthetic of metal nanoparticle is helpful to avoid the negative influence of physical and chemical process that is inappropriate for medical applications.
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