SUMMARYThe effects of concentrations of 10–30 mM/1 of CO2on the growth of psychrotrophic milk spoilage organisms were examined, both in untreated whole milk and in pure cultures ofPseudomonas fluorescensgrowing in skim-milk and broth. Spoilage of refrigerated untreated whole milk was found to be inhibited by the addition of CO2and the effect was enhanced with increasing concentrations of CO2and decreasing storage temperature. Experiments with pure cultures ofPs. fluorescensshowed that the inhibitory effects of CO2were not due to increased acidity or to displacement of dissolved O2, but to the presence of CO2per sewhich induced an increase in the duration of the lag phase of growth and had only a small effect on the logarithmic phase.

SUMMARYA commercial neutral proteinase was encapsulated in liposomes (multilamellar) and incorporated into cheese curd via the cheese milk. The milk proteins were protected from proteinase attack during curd manufacture. A significant proportion of the liposome-entrapped enzyme was retained in the curd and its subsequent release into the cheese matrix was inferred from an increased rate of β-casein breakdown.

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