In this paper we report this condition in 3 members of a Malay family. The unstable haemoglobin is structurally identical with Hb Koin first described by Pribilla (1962). We also report the results of enzyme studies of their red blood cells.
MethodsHaematological examinations were made by standard methods. Haemolysates were prepared from washed red blood cells by the addition of 1 vol water and 04 to 0 5 vol toluene. Haemoglobin electrophoresis was carried out in starch gel with tris-EDTA boric acid buffer at pH 8-0 and 8-6 and discontinuous tris-boric acid buffer at pH 9-5; agar gel electrophoresis, with citric acid buffer pH 6-2, and cellulose-acetate electrophoresis with
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