A new method is described for the isolation of subunits of the light-harvesting complex from Rhodospirillum rubrum (wild type and the G-9 mutant) in yields that approach 100%. The procedure involved treating membrane vesicles with ethylenediaminetetraacetic acid-Triton X-100 to remove components other than the light-harvesting complex and reaction center. In the preparation from wild-type cells, a benzene extraction was then employed to remove carotenoid and ubiquinone. The next step involved a careful addition of the detergent n-octyl beta-D-glucopyranoside, which resulted in a quantitative shift of the long-wavelength absorbance maximum from 873 to 820 nm. This latter complex was then separated from reaction centers by gel filtration on Sephadex G-100. The pigment-protein complex, now absorbing at 820 nm, contained two polypeptides of about 6-kilodalton molecular mass (referred to as alpha and beta) in a 1:1 ratio and two molecules of bacteriochlorophyll (BChl) for each alpha beta pair. This complex is much smaller in size than the original complex absorbing at 873 nm but probably is an associated form such as alpha 2 beta 2 X 4BChl or alpha 3 beta 3 X 6BChl. The 820-nm form could be completely shifted back to a form once again having a longer wavelength lambda max near 873 nm by decreasing the octyl glucoside concentration. Thus, the complex absorbing at 820 nm appears to be a subunit form of the original 873-nm complex.
We report a significant poleward surge in thermospheric winds at subauroral and midlatitudes following the 17–18 March 2015 great geomagnetic storm. This premidnight surge is preceded by strong westward winds. These disturbances were observed over three sites with geodetic latitudes 35–42°N in the American sector by Fabry‐Perot interferometers at 630 nm wavelength. Prior to the wind disturbances, subauroral polarization streams (SAPS) were measured by the Millstone Hill incoherent scatter radar between 20 and 02 UT. We identify the observed neutral wind variations as driven by SAPS, through a scenario where strong ion flows cause a westward neutral wind, subsequently establishing a poleward wind surge due to the poleward Coriolis force on that westward wind. These regional disturbances appear to have prevented the well‐known storm time equatorward wind surge from propagating into low latitudes, with the consequence that the classic disturbance dynamo mechanism failed to occur.
The first midlatitude conjugate thermospheric wind observations in the American sector showed various degrees of conjugacy between Palmer (64°S, 64°W, magnetic latitude (MLAT) 50°S) and Millstone Hill (42.82°N, 71.5°W, MLAT 53°N) under three different geomagnetic conditions (recovery after a substorm, moderately active, and quiet). The agreement with the National Center for Atmospheric Research's Thermosphere Ionosphere Electrodynamics General Circulation Model (TIEGCM) simulations also varies with the geomagnetic activity level. During substorm recovery, the observations at Palmer (PA) and Millstone Hill (MH) both showed strong westward zonal winds, which the standard TIEGCM greatly underestimated. Inadequate ion convection pattern size and lack of effect from Subauroral Polarization Streams (SAPS) may be the cause of the large discrepancy. The TIEGCM with a SAPS model produced stronger westward zonal winds near PA but did not change the zonal wind near MH. The empirical SAPS model needs further refinements. In general, there is better conjugacy with moderate geomagnetic activity levels. The TIEGCM also agrees better with the observations. Under geomagnetically quiet conditions, the meridional winds appear to be less conjugate. The agreement between the observations and model is reasonable. Optical conjugate observations are severely limited by the seasons and weather conditions in the two hemispheres. Yet they are necessary to understanding the thermospheric dynamics in the subauroral region and its relationship with geomagnetic activity levels. The comparisons with TIEGCM are necessary for future model improvements.
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