A method is described to determine hindgut CHo After establishing the total CH. production rate for a minimum of 24 h, the contents of the reticulorumen of the sheep were quantitatively removed through the rumen cannula and maintained at a temperature of 39'C with precautions Can. J. Anim. Sci. 64 (Suppl.): l7t-r72 (Sept. 1984) to exclude all O, entry. Pienaar et al. 1980). Both diets were supplemented with minerals while the CLS diet also contained fishmeal (7 Vo) and urea (0.25Vo).The diets were fed via the rumen cannula at a rate of 200 g every 4 h in order to maintain a stable fermentation pattern. In order to determine the repeatability and precision of measurements, hindgut CHo production rates were de-termined at 0800 and 1500 h on alternate days. Total CHo production was measured for a further period of 24 h after replacement of the reticuloruminal contents to determine the effect of emptying the reticulorumen on fermentation.The standard deviations for the average 4 hourly CH" production rates over the 48 h prior to emptying the reticulorumen indicate that the feeding procedure followed in this experiment can be used to maintain a relatively constant mean fermentation rate over periods between feedings. The methane production pattern between feeding periods (not shown in
Two complete diets, mordanted with chromium and mixed into the rumen were sampled from ileal digesta and feces. Marker passage studies showed that on maize cob leaves a significant part of the flow pattern, normally associated with mixing in the hindgut, originated anterior to the ileum while on lucerne it did not show the same pattern.Key words: Sheep, digesta, mixing compartments, marker passage Digesta flow kinetics in the whole digestive tract was first described by Blaxter et al. (1956) and later substantiated by Grovum and Williams (1973) For personal use only.
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