Aim: Employ of CRPs for utilization in tasar sericulture by needy organizations under various government schemes / developmental projects. Methodology: Community Resource Persons were nurtured under ISDS (Integrated Skill Development Scheme) of CSB and MKSP Tasar projects as a sector specialist cadre who works as specialist of knowing the cutting edge technologies of tasar culture and they will bring the theory of change further to unskilled community to build their skills and creating awareness about the new scientific practices of Tasar Sericulture and building their vision and understanding around communes. CRPs were trained collectively by PRADAN and CSB. Selection of beneficiaries, organizing them into Tasar Vikas Samities (TVSs), signing of agreement with beneficiaries, support for developing infrastructure, etc., during early period of the project, to get maximum project output and keep the tribal's interested in Tasar culture, utilization of the existing Tasar food plants in the forest areas/ raised under other schemes, Skill up-gradation, handholding support to the beneficiaries in creating activity groups were the primary activities achieved by engaging Community Resource Persons (CRP). Disease monitoring, technological support, etc., were monitored by these groups of resource person's. Results: A meticulous and customized evaluation programme of the CRPs was accomplished during 2022-23. A total of 479 CRPs nurtured under the special projects in the states of Jharkhand, Bihar, Chhattisgarh, Odisha and West Bengal, were accredited jointly by CTRTI Ranchi, Birsa Agricultural University Ranchi, KVKs and TDF-PRADAN Deoghar at field level. CRPs from 05 states covering 18 districts, 46 blocks, and 248 villages consisting 340 males & 139 females participated in evaluation & accreditation of CRPs. It was found that 36.74 percentage of CRP's had expertise in rearing activities followed by grainage and rearing (33.40%) and grainage (11.27%), however; only 8.56 % of the CRP's had their expertise in post cocoon technology. The results of assessment of Community Resource Persons (CRPs) based on different criteria revealed that the CRP's having expertise in both grainage & rearing scored 35.95 which is highest among the CRP's. Whereas lowest score of 30.65 was noticed with the CRP's who had expertise in Grainage. With respect to different criteria for assessment of the CRP's expertise in Tropical Tasar under the criteria of timeliness and sincerity; CRP's scored 7.07 out of 10 score followed by community mobilization (6.83) & on field technology support (6.79). Interpretation: As Tasar sector has attracted attention of many funding agencies in the recent past, keeping in view the need of horizontal expansion and shortage of manpower with the State Sericulture departments and others parallel institutions, CRPs can play a vital role based on their skill sets for further utilization by needy organizations under various government schemes/developmental projects. Key words: Community resource person, Extension, Tasar sector, Training
Semen collection, preservation and insemination are extensively used technique in higher organisms but in insects, sperm it is tricky. Owing the wild nature, even in natural condition A. mylitta faces coupling and insemination linked problems leading to low coupling percentage, unfertilized egg percentage and low fecundity etc. The insemination is having practical importance in breeding to transfer desired characters. In the present study hitches in semen collection and its insemination has been documented. The collection of semen from male moth of A. mylitta and insemination in females was performed. Collected semen was preserved at various temperatures (-20 0 C & -80 0 C and Cryo-planner with liquid nitrogen) showed 20-25% viability in sperm in contrast to the freshly collected semen viability 60-80%. Syringe-pipette-tips based probe was firm-up for insemination, although the hatching did not occurred in the inseminated moths but two positive sign was noticed (i) Eggs layed by inseminated moth-took more time to get depress in contrast to eggs layed by virgin female which indicates that insemination occurred in female but due some factors, hatching could not occurred (ii).The same was further verified by dissecting the inseminated females which showed the presence of sperms. To further explore the plausible reason for not getting eggs hatching, the freshly emerged male moth reproductive system was dissected and the sperms presence was checked which showed more sperms in the middle portion of the reproductive system. Based on this leads, the attempt was made to collect the sperm from the middle part of reproductive system and insemination in female moths but success not achieved in hatching. Although protein estimation, SDS-PAGE and microscopy was performed in order to envisage the changes in semen at morphological and protein level but correlation was not established. Present study indicated the unidentified crucial factor which caused hindrance in fertilization and hatching.
Aim: Analysis of chemical composition and assessment of biological potential of tasar exuviae extracts. Methodology: The biochemical components such as proteins, chitin, ash, fat, moisture, minerals and carbohydrate were estimated in the exuviae. After extraction, glowing pigments were characterized by UV-Vis and fluorescent spectra, and profiling of polyphenolic and flavonoids, free radical scavenging assay, antibacterial and anticancer potential were evaluated. Results: The proximate components such as proteins, chitin, ash, fat, moisture, and carbohydrate contents were 62, 11.52, 10.57, 3.9, 3.1 and 0.9%, respectively. Further, among different elements, calcium level was higher than other elements such as magnesium, manganese, copper, zinc and sodium. The methanolic extract exhibited free radical scavenging and anti microbial potential in concentration dependent manner. In addition to these, glowing compounds prominent cytotoxic effect against breast cancer cell lines viz. MCF7, BT474 and SKBR3. Interpretation: The action mechanism of extract could be probably due to the presence of yellow flavonoids (luteolin, catechin, epicatechin and quercetin) and phenolic acids (p-coumaric acid, gentisic acid and gallic acid), which glows during exposure of UV light and having biological potentials. Key words: Anticancer, Antheraea mylitta, Tasar exuviae
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