British and British x Continental steers (n = 560; initial BW = 339.4 +/- 1.76 kg) were used in a serial slaughter study with a completely random design to evaluate effects of zilpaterol hydrochloride (ZH; 8.33 mg/kg of dietary DM basis) on performance and carcass characteristics. Treatments were arranged in a 4 x 4 factorial (112 pens; 7 pens/treatment; 5 steers/pen) and included duration of ZH feeding (0, 20, 30, or 40 d before slaughter plus a 3-d ZH withdrawal period) and days on feed (DOF) before slaughter (136, 157, 177, and 198 d). No duration of ZH feeding x slaughter group interactions were detected for the performance measurements (P > 0.10). Final BW did not differ (P = 0.15) between the 0-d group and the average of the 3 ZH groups, but ADG was greater for the average of the 3 ZH groups during the period in which ZH diets were fed (P < 0.01) and for the overall feeding period (P = 0.05). As duration of ZH feeding increased, DMI decreased (P = 0.01) and G:F increased linearly (P < 0.01). With the exception of KPH (P = 0.022), no duration of ZH feeding x slaughter group interactions (P > 0.10) were detected for carcass characteristics. Regardless of the duration of ZH feeding, cattle fed ZH had greater HCW (P < 0.01), greater dressing percent (P < 0.01), less 12th-rib fat (P < 0.01), larger LM area (P < 0.01), less KPH (P = 0.03), and lower yield grade (P < 0.01) than the 0-d cattle. The 0-d group had greater marbling scores (P < 0.01) than cattle fed ZH diets, with a tendency for a linear decrease in marbling score (P = 0.10) as duration of ZH feeding was extended. A greater percentage of carcasses in the 0-d group graded USDA Choice or greater (P < 0.01) than in the 3 ZH groups, whereas the percentage of Select carcasses was greater (P = 0.01) for the 3 ZH groups. From d 0 to end (P = 0.04) and during the last 43 d on feed (P < 0.01), ADG responded quadratically to DOF before slaughter. No differences were detected among slaughter groups for DMI for the entire trial period; however, a quadratic response (P = 0.02) was observed for the final 43 d before slaughter. A quadratic response was also detected for the final 43 d before slaughter (P < 0.01) and from d 0 to end (P = 0.02) for G:F. Final BW, HCW, dressing percent, and 12th-rib fat increased linearly (P < 0.01) as DOF before slaughter increased. Our results indicate that no substantial effects on performance and carcass measurements were observed when ZH was fed for 30 or 40 d as opposed to 20 d, and that effects of ZH generally did not interact with DOF before slaughter.
Two experiments evaluated effects of ractopamine hydrochloride (RAC) on performance, intake patterns, and acid-base balance of feedlot cattle. In Exp. 1, 360 crossbred steers (Brangus, British, and British x Continental breeding; initial BW = 545 kg) were used in a study with a 3 x 3 factorial design to study the effects of dose [0, 100, or 200 mg/(steer x d) of RAC] and duration (28, 35, or 42 d) of feeding of RAC in a randomized complete block design (9 treatments, 8 pens/treatment). No dose x duration interactions were detected (P > 0.10). As RAC dose increased, final BW (FBW; P = 0.01), ADG (P < 0.01), and G:F (P < 0.01) increased linearly. As duration of feeding increased, ADG increased quadratically (P = 0.04), with tendencies for quadratic effects for FBW (P = 0.06), DMI (P = 0.07), and G:F (P = 0.09). Hot carcass weight increased linearly (P = 0.02) as dose of RAC increased. Thus, increasing the dose of RAC from 0 to 200 mg/(steer x d) and the duration of feeding from 28 to 42 d improved feedlot performance, although quadratic responses for duration of feeding indicated little improvement as the duration was extended from 35 to 42 d. In Exp. 2, 12 crossbred beef steers (BW = 593 kg) were used in a completely random design to evaluate the effects of RAC [0 or 200 mg/(steer x d) for 30 d; 6 steers/treatment] on rate of intake, daily variation in intake patterns, and acid-base balance. To assess intake patterns, absolute values of daily deviations in feed delivered to each steer relative to the total quantity of feed delivered were analyzed as repeated measures. There were no differences (P > 0.10) in feedlot performance, urine pH, blood gas measurements, or variation in intake patterns between RAC and control cattle, but steers fed RAC had increased (P = 0.04) LM area, decreased (P = 0.03) yield grade, and increased (P < 0.10) time to consume 50 and 75% of daily intake relative to control steers. Our results suggest that feeding RAC for 35 d at 200 mg/(steer x d) provided optimal performance, and no effects on acid-base balance or variation in intake patterns of finishing steers were noted with RAC fed at 200 mg/(steer x d) over a 30-d period.
The effects of a live yeast supplement [Saccharomyces cerevisiae subspecies boulardii CNCM I-1079; ProTernative Stress Formula (PTSF) yeast, Ivy Natural Solutions, Overland Park, KS] on DMI, performance, and health of beef cattle were evaluated in 3 experiments. In Exp. 1, a pilot study was conducted with 10 healthy beef steers fed a 65% concentrate diet to evaluate the effects of florfenicol (s.c. in the neck vs. sterile water injection) on DMI. Steers injected with florfenicol had 15.6 (P = 0.092) and 22.2% (P = 0.015) decreases in DMI compared with controls on the day of and day after injection, respectively, with no differences for the remainder of the 7-d period. In the main study of Exp. 1, healthy beef steers (6 pens of 5 steers each/treatment) were fed the control or PTSF yeast diets (0.5 g of yeast x steer(-1) x d(-1)) for 5 d before being injected s.c. with florfenicol. Compared with the 5 d before injection, DMI decreased after injection, but it did not differ (P > 0.66) between treatments on the day of and day after injection. By the second day after injection, DMI tended (P = 0.107) to increase for steers fed PTSF yeast vs. control steers, with a trend for a similar pattern on the third day after injection (P = 0.197). No differences were noted between treatments for the remainder of the 7-d period or for the subsequent 2 wk. In Exp. 2, 3 loads of beef heifers (277 heifers; average initial BW = 230.3 kg) were shipped from auction barns and assigned randomly to 1 of 2 treatments (5 pens/treatment in each load) during 35-d receiving periods: 1) control = 65% concentrate receiving diet; or 2) PTSF yeast = 65% concentrate receiving diet with PTSF yeast added to supply 0.5 g of yeast x heifer(-1) x d(-1). All heifers were treated with florfenicol on arrival, and PTSF yeast heifers received approximately 1 g of yeast via an oral paste at the time of processing. Averaged over the 3 loads, treatments did not affect (P > or = 0.12) DMI, ADG, or G:F during the 35-d period, but the percentage of cattle treated once or more for bovine respiratory disease (BRD) was greater for control (P = 0.04) than for PTSF yeast heifers (24.0 vs. 13.78% respectively). In Exp. 3, 2 loads of beef heifers (180 heifers; average initial BW = 209.0 kg) that were not treated with antibiotic at the time of arrival processing were fed a 70% concentrate receiving diet and assigned the same 2 treatments as in Exp. 2. No differences (P > 0.72) were noted between treatments in ADG, DMI, and G:F for the 35-d receiving period, and BRD morbidity pooled across loads did not differ between treatments (40.2 vs. 33.1% for control vs. PTSF yeast). Providing PTSF yeast in an oral paste at the time of processing combined with the addition of 0.5 g of yeast x animal(-1) x d(-1) in the diet had little effect on receiving period performance; however, it decreased BRD morbidity in heifers given florfenicol on arrival but was without effect on BRD morbidity in heifers that did not receive a prophylactic antibiotic.
Two experiments examined the effects of a saccharin-based artificial sweetener (Sucram) on health, performance, and dietary preference of feedlot cattle. In Exp. 1, 200 steer calves (initial BW = 190.4 +/- 1.47 kg) were fed a 65% concentrate diet supplemented with or without 200 mg of Sucram/kg (DM basis) during a 56-d receiving-growing period. Feeding Sucram did not affect overall (P = 0.19) DMI; however, from d 29 to 56, there was a trend (P = 0.10) for increased DMI with Sucram (5.71 vs. 6.02 kg/d, respectively). From d 0 to 28 and d 0 to 56, there were trends (P = 0.11 and 0.12, respectively) for increased ADG and for increased d-56 BW (P = 0.07) for calves fed Sucram. No differences were detected (P = 0.82) for receiving (REC) period morbidity. During the finishing (FIN) period, 180 steers from the REC period were assigned (9 pens/treatment in a 2 x 2 factorial design) to the following treatments: 1) control REC/control FIN; 2) control REC/Sucram FIN; 3) Sucram REC/control FIN; and 4) Sucram REC/ Sucram FIN. Over the FIN period, ADG tended (P = 0.12) to be greater for Sucram; however, carcass-adjusted ADG did not differ among treatments. Daily DMI was affected by a REC x FIN interaction (P = 0.08), which was the result of greater DMI by cattle in the Sucram REC/Sucram FIN treatment and decreased DMI by cattle in the Sucram REC/control FIN treatment. In general, changes in carcass characteristics were minor. In Exp. 2, 12 steers (initial BW = 395.6 +/- 6.17 kg) were used in a simultaneously replicated 3 x 3 Latin square preference test. Each square consisted of 3 pens, with 2 steers/pen, and 3 time periods. Bunks had dividers at their midpoint, and equal quantities of diet (as-fed basis) were delivered randomly on either side of the divider daily. Treatments were: 1) control; 2) Sucram = basal diet supplemented with 200 mg of Sucram/kg of DM; and 3) choice = control and Sucram on separate sides of the divider. Dietary preference differed on d 1 (P = 0.01) and d 3 (P = 0.02) for control vs. choice and Sucram vs. choice, with the choice group consuming 0.49 and 1.72 kg of DM more of the Sucram diet than the control diet, respectively. This effect, however, was not consistent across days, and average DMI did not differ (P = 0.81) among treatments. Addition of Sucram to the diet of newly received cattle tended to increase receiving period ADG; however, its effects on morbidity, finishing performance, and dietary preference were limited.
Effects of varying bulk densities of steam-flaked corn (SFC) and level of inclusion of roughage in feedlot diets were evaluated in 3 experiments. In Exp. 1, a total of 128 beef steers were used in a 2 x 2 factorial arrangement to evaluate the effects of bulk density of SFC (335 or 386 g/L) and roughage concentration (6 or 10% ground alfalfa hay, DM basis) on performance and carcass characteristics. No interactions were observed between bulk density and roughage concentration for performance data. From d 0 to the end, cattle fed the 335 g/L SFC had greater overall G:F (P = 0.04) than those fed the 386 g/L SFC, with tendencies (P < 0.10) for improved G:F with the lighter flake weight evident at all 35-d intervals throughout the feeding period. Dry matter intake was less for cattle fed 6 vs. 10% roughage from d 0 to 35 (P = 0.03) and d 0 to 70 (P = 0.05), but not for the overall feeding period. Feeding 6 vs. 10% ground alfalfa as the roughage source tended (P = 0.09) to improve overall G:F. Treatment effects on carcass measurements were generally not significant (P > 0.20). In Exp. 2, the effects of bulk density of SFC (283, 335, or 386 g/L) and 6 or 10% ground alfalfa hay on IVDMD and in vitro pH were evaluated at 6, 12, 18, and 24 h of incubation. With a reduced-strength buffer in vitro fermentation system, pH increased (P < 0.01) with increasing bulk density at 6 and 12 h, and IVDMD decreased (P < 0.03) as bulk density increased. In contrast, in a normal-strength buffer system, there were no treatment differences (P > 0.23) for IVDMD. In Exp. 3, two diets that varied in bulk density of SFC and roughage concentration (335 g/L SFC with 6% alfalfa hay vs. 386 g/L SFC with 10% alfalfa hay) were compared for their effects on the pattern of feed intake and the acid-base balance in Holstein steers (12/treatment). No differences (P > 0.10) between treatments were noted for blood gases or urine pH; however, day effects (P < 0.02) were detected for blood pH, partial pressure of CO(2), and urine pH, which generally decreased (P < 0.05) with an increasing time on feed. The 2 treatments had little effect on the pattern of feed intake within the sampling days, with the exception that the 386 g/L SFC with 10% alfalfa hay diet increased (P < 0.05) the percentage of total DMI consumed at 1 and 6 h after feeding on d 14. Within the ranges of bulk density and roughage level studied, 335 g/L SFC with 6% alfalfa hay yielded the optimal animal performance, with limited effects on in vitro fermentation and the acid-base balance.
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