Acid Hydrolysis: (A) dZ-a-Aminononanoic Acid.-A solution of 22.2 g. of diethyl re-heptylacetamidomalonate in 100 ml. of concentrated hydrochloric acid was refluxed for seven hours and then cooled to 0°. There was thus precipitated a mass of shiny pearly plates. This material was collected by filtration and dried yielding 10.7 g. of amino acid hydrochloride. The crude product was dissolved in a mixture of ethanol and water (1:1) and the amino acid precipitated by the addition of 15 ml. of pyridine. The product was filtered, washed and air dried; yield 6.7 g. (55%). It was further purified with little or no loss by solution in 500 ml. of water containing 15 ml. of 10% sodium hydroxide, treating with charcoal, filtering and adding excess acetic acid to precipitate the amino acid. The pure compound melted at 270-273°( dec.).Anal. Caled, for CsHuCbX: X, 8.08. Found: X, 7.83. This amino acid could be isolated equally well by the use of ammonium hydroxide in place of pyridine since it is not very soluble in water.(Compare the isolation of dlleucine.4d) Procedure (B) is preferable for lower molecular weight amino acids. ucts have been hydrolyzed to amino acids which have been characterized through the benzoyl derivatives.Rensselaer, Xt. Y.
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