The tiger quoll or spotted-tailed quoll Dasyurus maculatus, a nationally threatened species, is the largest surviving carnivorous marsupial on mainland Australia and the sole surviving member of the genus in south-eastern mainland Australia. Live-trapping and radio-tracking studies at three sites in south-eastern Australia found that tiger quolls were solitary, except when mating, and that male and female tiger quolls occupied very large home ranges, both during and after the breeding season. The mean size of the home range of male tiger quolls (minimum convex polygon (MCP) 1755.4 ha, kernel 3761.7 ha) was significantly larger than the mean size of the home range of females (MCP 495.9 ha, kernel 1113.0 ha). Adult female tiger quolls displayed intrasexual territoriality throughout the year, but they seemed to tolerate the presence of female offspring. Male tiger quolls were not territorial and their home ranges overlapped both with other males and with females throughout the year. Males were recorded regularly moving back and forth between a number of female territories during the breeding season. The spatial organization of the tiger quoll recorded in this study differs markedly from that of the eastern quoll Dasyurus viverrinus and the Tasmanian devil Sarcophilus harrisii, but is similar to that of the western quoll D. geoffroii, northern quoll D. hallucatus and brush-tailed phascogale Phascogale tapoatafa. The spatial organization and home-range size of the tiger quoll dictate that conservation of the species requires management across all land tenures at the landscape level.

Analysis of 338 tiger quoll scats from tableland moist forest in south-eastern New South Wales found that the greater glider was the major prey species occurring in 54.1% of scats and contributing an estimated 51.01% of the biomass consumed by tiger quolls. Medium-sized (0.5–5 kg) mammals were the most important prey group by occurrence (53.9%), frequency (66.0%) and estimated biomass contribution to diet (62.93%). Other medium-sized prey taken by tiger quolls included: long-nosed bandicoot, rabbit, brushtail possum and ringtail possum. Macropods and wombats were also present in the scats and had been presumably taken as carrion. There was no significant difference in the diets of male and female tiger quolls. Tiger quolls were recorded hunting greater gliders in their tree hollows during the day, hunting rabbits in their burrows both during the day and at night and were observed eating road-killed macropods and wombats around dawn and dusk.

Habitat use by tiger quolls was investigated at three sites in south-eastern Australia through trapping and radio-tracking. Habitat complexity was measured in each of the habitat types present. Prey densities were determined from spotlighting transects in each habitat type. Habitat utilization differed between sites, but gullies and drainage lines were used significantly more than availability at each site. Escarpment was used more than availability at Suggan Buggan and ridges were used in proportion to availability at Tallaganda. Mid-slopes were used significantly less than availability at all sites. Habitat use was significantly related with prey densities at most sites. Rock dens appeared to be preferred over log dens, resulting in the use of ridges when complex rocky outcrops, suitable for den sites, were present. Habitat complexity was high in gullies/lower slopes/riparian flats, moderate on mid-slopes and low to moderate on upper slopes/ridges. High prey densities and the presence of preferred den substrates were the two factors that influenced habitat use by quolls in this study.

Den use was investigated by radio-tracking 28 spotted-tailed quolls (Dasyurus maculatus) at three sites in south-eastern Australia. Dens were recorded in rock crevices, caves and boulder tumbles, hollow logs, tree hollows and windrows and in small wombat (Vombatus ursinus) and rabbit (Oryctolagus cuniculus) burrows. Two D. maculatus were recorded using more than 15 dens during this study. Rock dens appeared to be preferred over hollow log dens. D. maculatus denned alone, except when rearing young and possibly during mating. Females used a number of maternal dens while rearing young, some maternal dens were used in consecutive years.